2O7M
The C-terminal loop of the homing endonuclease I-CreI is essential for DNA binding and cleavage. Identification of a novel site for specificity engineering in the I-CreI scaffold
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A, B (A, B) | DNA endonuclease I-CreI | polymer | 156 | 17934.6 | 2 | UniProt (P05725) Pfam (PF00961) | Chlamydomonas reinhardtii | LAGLIDAG HOMING ENDONUCLEASE, 23S rRNA intron protein |
| 2 | C, D (A, B) | water | water | 18.0 | 262 | Chemie (HOH) |
Sequence modifications
A, B: 1 - 156 (UniProt: P05725)
| PDB | External Database | Details |
|---|---|---|
| Thr 42 | Ala 42 | conflict |
| Glu 110 | Trp 110 | conflict |
| Gln 111 | Arg 111 | conflict |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 2 |
| Total formula weight | 35869.3 | |
| All* | Total formula weight | 35869.3 |
