2O3E

Crystal structure of engineered neurolysin with thimet oligopeptidase specificity for neurotensin cleavage site.

2O3E の概要

• エントリーDOI: 10.2210/pdb2o3e/pdb
• 関連するPDBエントリー: 1I1I
• 分子名称: Neurolysin, ZINC ION (3 entities in total)
• 機能のキーワード: thermolysin-like domain, substrate-binding channel, hydrolase
• 由来する生物種: Rattus norvegicus (Norway rat)
• 細胞内の位置: Mitochondrion intermembrane space: P42676
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 77710.05

構造登録者

Rodgers, D.W.,Lim, E.J. (登録日: 2006-12-01, 公開日: 2007-01-23, 最終更新日: 2023-08-30)

主引用文献

Lim, E.J.,Sampath, S.,Coll-Rodriguez, J.,Schmidt, J.,Ray, K.,Rodgers, D.W.
Swapping the substrate specificities of the neuropeptidases neurolysin and thimet oligopeptidase.
J.Biol.Chem., 282:9722-9732, 2007

PubMed Abstract: Thimet oligopeptidase (EC 3.4.24.15) and neurolysin (EC 3.4.24.16) are closely related zinc-dependent metallopeptidases that metabolize small bioactive peptides. They cleave many substrates at the same sites, but they recognize different positions on others, including neurotensin, a 13-residue peptide involved in modulation of dopaminergic circuits, pain perception, and thermoregulation. On the basis of crystal structures and previous mapping studies, four sites (Glu-469/Arg-470, Met-490/Arg-491, His-495/Asn-496, and Arg-498/Thr-499; thimet oligopeptidase residues listed first) in their substrate-binding channels appear positioned to account for differences in specificity. Thimet oligopeptidase mutated so that neurolysin residues are at all four positions cleaves neurotensin at the neurolysin site, and the reverse mutations in neurolysin switch hydrolysis to the thimet oligopeptidase site. Using a series of constructs mutated at just three of the sites, it was determined that mutations at only two (Glu-469/Arg-470 and Arg-498/Thr-499) are required to swap specificity, a result that was confirmed by testing the two-mutant constructs. If only either one of the two sites is mutated in thimet oligopeptidase, then the enzyme cleaves almost equally at the two hydrolysis positions. Crystal structures of both two-mutant constructs show that the mutations do not perturb local structure, but side chain conformations at the Arg-498/Thr-499 position differ from those of the mimicked enzyme. A model for differential recognition of neurotensin based on differences in surface charge distribution in the substrate binding sites is proposed. The model is supported by the finding that reducing the positive charge on the peptide results in cleavage at both hydrolysis sites.

PubMed: 17251185
DOI: 10.1074/jbc.M609897200

実験手法

X-RAY DIFFRACTION (2.2 Å)