2O01

The Structure of a plant photosystem I supercomplex at 3.4 Angstrom resolution

Summary for 2O01

• Entry DOI: 10.2210/pdb2o01/pdb
• Related: 1QZV
• Descriptor: Photosystem I P700 chlorophyll a apoprotein A1, Photosystem I reaction center subunit IX, Photosystem I reaction center subunit psaK, chloroplast, ... (21 entities in total)
• Functional Keywords: membranal super complex, photosynthesis
• Biological source: Pisum sativum (pea); More
• Cellular location: Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein (By similarity): P05310 P05311; Plastid, chloroplast thylakoid membrane; Single-pass membrane protein (By similarity): P17230 P22179 P17227; Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein (Probable): Q41385 P12357; Plastid, chloroplast thylakoid membrane; Multi-pass membrane protein: Q01667 Q9SQL2; Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side (By similarity): P10793; Plastid, chloroplast thylakoid membrane; Peripheral membrane protein; Stromal side: P12353; Plastid, chloroplast thylakoid membrane; Peripheral membrane protein (By similarity): Q9S831; Plastid, chloroplast thylakoid lumen: P12355
• Total number of polymer chains: 17
• Total formula weight: 504246.11

Authors

Amunts, A.,Drory, O.,Nelson, N. (deposition date: 2006-11-27, release date: 2007-05-08, Last modification date: 2023-08-30)

Primary citation

Amunts, A.,Drory, O.,Nelson, N.
The structure of a plant photosystem I supercomplex at 3.4 A resolution.
Nature, 447:58-63, 2007

PubMed Abstract: All higher organisms on Earth receive energy directly or indirectly from oxygenic photosynthesis performed by plants, green algae and cyanobacteria. Photosystem I (PSI) is a supercomplex of a reaction centre and light-harvesting complexes. It generates the most negative redox potential in nature, and thus largely determines the global amount of enthalpy in living systems. We report the structure of plant PSI at 3.4 A resolution, revealing 17 protein subunits. PsaN was identified in the luminal side of the supercomplex, and most of the amino acids in the reaction centre were traced. The crystal structure of PSI provides a picture at near atomic detail of 11 out of 12 protein subunits of the reaction centre. At this level, 168 chlorophylls (65 assigned with orientations for Q(x) and Q(y) transition dipole moments), 2 phylloquinones, 3 Fe(4)S(4) clusters and 5 carotenoids are described. This structural information extends the understanding of the most efficient nano-photochemical machine in nature.

PubMed: 17476261
DOI: 10.1038/nature05687

Experimental method

X-RAY DIFFRACTION (3.4 Å)