2O01
The Structure of a plant photosystem I supercomplex at 3.4 Angstrom resolution
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-2 |
| Synchrotron site | ESRF |
| Beamline | ID23-2 |
| Temperature [K] | 100 |
| Wavelength(s) | 0.875 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 120.054, 188.784, 127.518 |
| Unit cell angles | 90.00, 95.19, 90.00 |
Refinement procedure
| Resolution | 40.000 - 3.400 |
| R-factor | 0.351 |
| Rwork | 0.348 |
| R-free | 0.40900 |
| Structure solution method | SAD |
| Starting model (for MR) | 1qzv |
| RMSD bond length | 0.017 |
| RMSD bond angle | 4.101 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | DM |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.520 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.140 | 0.475 |
| Number of reflections | 70551 | |
| <I/σ(I)> | 16.41 | 3.4 |
| Completeness [%] | 91.1 | 78.9 |
| Redundancy | 3.6 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6 | 278 | pH 6.0, VAPOR DIFFUSION, SITTING DROP, temperature 278K |
