2NVU

Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex

Summary for 2NVU

• Entry DOI: 10.2210/pdb2nvu/pdb
• Descriptor: NEDD8-activating enzyme E1 regulatory subunit, Maltose binding protein/NEDD8-activating enzyme E1 catalytic subunit chimera, NEDD8-conjugating enzyme Ubc12, ... (8 entities in total)
• Functional Keywords: multifunction macromolecular complex, ubiquitin, nedd8, e1, e2, atp, conformational change, thioester, switch, adenylation, protein turnover, ligase
• Biological source: Homo sapiens (human); More
• Cellular location: Cell membrane: Q13564; Nucleus: Q15843
• Total number of polymer chains: 5
• Total formula weight: 188893.13

Authors

Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A. (deposition date: 2006-11-13, release date: 2007-01-30, Last modification date: 2023-08-30)

• Primary citation: Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A.; Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.; Nature, 445:394-398, 2007; PubMed: 17220875; DOI: 10.1038/nature05490

Experimental method

X-RAY DIFFRACTION (2.8 Å)