2NVU

Structure of APPBP1-UBA3~NEDD8-NEDD8-MgATP-Ubc12(C111A), a trapped ubiquitin-like protein activation complex

2NVU の概要

• エントリーDOI: 10.2210/pdb2nvu/pdb
• 分子名称: NEDD8-activating enzyme E1 regulatory subunit, Maltose binding protein/NEDD8-activating enzyme E1 catalytic subunit chimera, NEDD8-conjugating enzyme Ubc12, ... (8 entities in total)
• 機能のキーワード: multifunction macromolecular complex, ubiquitin, nedd8, e1, e2, atp, conformational change, thioester, switch, adenylation, protein turnover, ligase
• 由来する生物種: Homo sapiens (human); 詳細
• 細胞内の位置: Cell membrane: Q13564; Nucleus: Q15843
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 188893.13

構造登録者

Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A. (登録日: 2006-11-13, 公開日: 2007-01-30, 最終更新日: 2023-08-30)

主引用文献

Huang, D.T.,Hunt, H.W.,Zhuang, M.,Ohi, M.D.,Holton, J.M.,Schulman, B.A.
Basis for a ubiquitin-like protein thioester switch toggling E1-E2 affinity.
Nature, 445:394-398, 2007

PubMed Abstract: Ubiquitin-like proteins (UBLs) are conjugated by dynamic E1-E2-E3 enzyme cascades. E1 enzymes activate UBLs by catalysing UBL carboxy-terminal adenylation, forming a covalent E1 throught UBL thioester intermediate, and generating a thioester-linked E2 throught UBL product, which must be released for subsequent reactions. Here we report the structural analysis of a trapped UBL activation complex for the human NEDD8 pathway, containing NEDD8's heterodimeric E1 (APPBP1-UBA3), two NEDD8s (one thioester-linked to E1, one noncovalently associated for adenylation), a catalytically inactive E2 (Ubc12), and MgATP. The results suggest that a thioester switch toggles E1-E2 affinities. Two E2 binding sites depend on NEDD8 being thioester-linked to E1. One is unmasked by a striking E1 conformational change. The other comes directly from the thioester-bound NEDD8. After NEDD8 transfer to E2, reversion to an alternate E1 conformation would facilitate release of the E2 throught NEDD8 thioester product. Thus, transferring the UBL's thioester linkage between successive conjugation enzymes can induce conformational changes and alter interaction networks to drive consecutive steps in UBL cascades.

PubMed: 17220875
DOI: 10.1038/nature05490

実験手法

X-RAY DIFFRACTION (2.8 Å)