2NS7

How an in vitro selected peptide mimics the antibiotic tetracycline to induce TET repressor

2NS7 の概要

• エントリーDOI: 10.2210/pdb2ns7/pdb
• 関連するPDBエントリー: 1QPI 2NS8 2TCT
• 分子名称: Tetracycline repressor protein (1 entity in total)
• 機能のキーワード: transcription regulator, helix-turn-helix, transcription
• 由来する生物種: Escherichia coli; 詳細
• タンパク質・核酸の鎖数: 4
• 化学式量合計: 94126.65

構造登録者

Luckner, S.R.,Klotzsche, M.,Berens, C.,Hillen, W.,Muller, Y.A. (登録日: 2006-11-03, 公開日: 2007-07-24, 最終更新日: 2023-10-25)

主引用文献

Luckner, S.R.,Klotzsche, M.,Berens, C.,Hillen, W.,Muller, Y.A.
How an agonist peptide mimics the antibiotic tetracycline to induce Tet-repressor
J.Mol.Biol., 368:780-790, 2007

PubMed Abstract: A 16-residue peptide, called Tip, induces the tetracycline repressor TetR as efficiently as the antibiotic tetracycline when fused to the N or C terminus of another protein. This is unusual because the majority of in vitro selected peptides, such as Tip, inhibit protein function, and agonist peptides are only rarely identified. We elucidated the atomic mechanism of TetR induction by Tip from crystal structures of TetR in complex with Tip and of free TetR. Peptide induction ultimately results in the same movements of DNA reading heads, but Tip accomplishes this by very different molecular interactions from tetracycline involving important contacts to the TetR surface. As a direct consequence, an alternate pathway of allostery becomes possible that makes ample use of intersubunit interactions. For the first time it is possible to show in atomic detail how a small molecule controlled bacterial transcription factor such as TetR becomes responsive to protein-protein interactions, characteristic of gene transcription regulation in higher organisms.

PubMed: 17374541
DOI: 10.1016/j.jmb.2007.02.030

実験手法

X-RAY DIFFRACTION (2.4 Å)