2MXP
Solution structure of NDP52 ubiquitin-binding zinc finger
Summary for 2MXP
| Entry DOI | 10.2210/pdb2mxp/pdb |
| Related | 4XKL |
| NMR Information | BMRB: 25423 |
| Descriptor | Calcium-binding and coiled-coil domain-containing protein 2, ZINC ION (2 entities in total) |
| Functional Keywords | zinc finger, ndp52, ubiquitin-binding, c2h2-type, metal binding protein |
| Biological source | Homo sapiens (human) |
| Cellular location | Cytoplasm, perinuclear region : Q13137 |
| Total number of polymer chains | 1 |
| Total formula weight | 3952.95 |
| Authors | |
| Primary citation | Xie, X.,Li, F.,Wang, Y.,Wang, Y.,Lin, Z.,Cheng, X.,Liu, J.,Chen, C.,Pan, L. Molecular basis of ubiquitin recognition by the autophagy receptor CALCOCO2 Autophagy, 11:1775-1789, 2015 Cited by PubMed Abstract: The autophagy receptor CALCOCO2/NDP52 functions as a bridging adaptor and plays an essential role in the selective autophagic degradation of invading pathogens by specifically recognizing ubiquitin-coated intracellular pathogens and subsequently targeting them to the autophagic machinery; thereby it is required for innate immune defense against a range of infectious pathogens in mammals. However, the mechanistic basis underlying CALCOCO2-mediated specific recognition of ubiqutinated pathogens is still unknown. Here, using biochemical and structural analyses, we demonstrated that the cargo-binding region of CALCOCO2 contains a dynamic unconventional zinc finger as well as a C2H2-type zinc-finger, and only the C2H2-type zinc finger specifically recognizes mono-ubiquitin or poly-ubiquitin chains. In addition to elucidating the specific ubiquitin recognition mechanism of CALCOCO2, the structure of the CALCOCO2 C2H2-type zinc finger in complex with mono-ubiquitin also uncovers a unique zinc finger-binding mode for ubiquitin. Our findings provide mechanistic insight into how CALCOCO2 targets ubiquitin-decorated pathogens for autophagic degradations. PubMed: 26506893DOI: 10.1080/15548627.2015.1082025 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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