2MS7

High-resolution solid-state NMR structure of the helical signal transduction filament MAVS CARD

Summary for 2MS7

• Entry DOI: 10.2210/pdb2ms7/pdb
• Related: 2MS7
• NMR Information: BMRB: 25076
• Descriptor: Mitochondrial antiviral-signaling protein (1 entity in total)
• Functional Keywords: mavs card filament, protein binding
• Biological source: Homo sapiens (human)
• Cellular location: Mitochondrion outer membrane: Q7Z434
• Total number of polymer chains: 21
• Total formula weight: 248164.62

Authors

He, L.,Bardiaux, B.,Spehr, J.,Luehrs, T.,Ritter, C. (deposition date: 2014-07-25, release date: 2015-09-02, Last modification date: 2024-05-01)

Primary citation

He, L.,Bardiaux, B.,Ahmed, M.,Spehr, J.,Konig, R.,Lunsdorf, H.,Rand, U.,Luhrs, T.,Ritter, C.
Structure determination of helical filaments by solid-state NMR spectroscopy.
Proc.Natl.Acad.Sci.USA, 113:E272-E281, 2016

PubMed Abstract: The controlled formation of filamentous protein complexes plays a crucial role in many biological systems and represents an emerging paradigm in signal transduction. The mitochondrial antiviral signaling protein (MAVS) is a central signal transduction hub in innate immunity that is activated by a receptor-induced conversion into helical superstructures (filaments) assembled from its globular caspase activation and recruitment domain. Solid-state NMR (ssNMR) spectroscopy has become one of the most powerful techniques for atomic resolution structures of protein fibrils. However, for helical filaments, the determination of the correct symmetry parameters has remained a significant hurdle for any structural technique and could thus far not be precisely derived from ssNMR data. Here, we solved the atomic resolution structure of helical MAVS(CARD) filaments exclusively from ssNMR data. We present a generally applicable approach that systematically explores the helical symmetry space by efficient modeling of the helical structure restrained by interprotomer ssNMR distance restraints. Together with classical automated NMR structure calculation, this allowed us to faithfully determine the symmetry that defines the entire assembly. To validate our structure, we probed the protomer arrangement by solvent paramagnetic resonance enhancement, analysis of chemical shift differences relative to the solution NMR structure of the monomer, and mutagenesis. We provide detailed information on the atomic contacts that determine filament stability and describe mechanistic details on the formation of signaling-competent MAVS filaments from inactive monomers.

PubMed: 26733681
DOI: 10.1073/pnas.1513119113

Experimental method

SOLID-STATE NMR