2MP1

Solution structure of the human chemokine CCL19

Summary for 2MP1

• Entry DOI: 10.2210/pdb2mp1/pdb
• NMR Information: BMRB: 19960
• Descriptor: C-C motif chemokine 19 (1 entity in total)
• Functional Keywords: chemokines, chemokine receptors, ccl19, ccl21, ccr7, signaling protein
• Biological source: Homo sapiens (human)
• Cellular location: Secreted: Q99731
• Total number of polymer chains: 1
• Total formula weight: 8814.28

Authors

Veldkamp, C.T.,Peterson, F.C.,Gabel-Eissens, S.J.,Gillitzer, M.L. (deposition date: 2014-05-09, release date: 2015-06-10, Last modification date: 2024-10-30)

Primary citation

Veldkamp, C.T.,Kiermaier, E.,Gabel-Eissens, S.J.,Gillitzer, M.L.,Lippner, D.R.,DiSilvio, F.A.,Mueller, C.J.,Wantuch, P.L.,Chaffee, G.R.,Famiglietti, M.W.,Zgoba, D.M.,Bailey, A.A.,Bah, Y.,Engebretson, S.J.,Graupner, D.R.,Lackner, E.R.,LaRosa, V.D.,Medeiros, T.,Olson, M.L.,Phillips, A.J.,Pyles, H.,Richard, A.M.,Schoeller, S.J.,Touzeau, B.,Williams, L.G.,Sixt, M.,Peterson, F.C.
Solution Structure of CCL19 and Identification of Overlapping CCR7 and PSGL-1 Binding Sites.
Biochemistry, 54:4163-4166, 2015

PubMed Abstract: CCL19 and CCL21 are chemokines involved in the trafficking of immune cells, particularly within the lymphatic system, through activation of CCR7. Concurrent expression of PSGL-1 and CCR7 in naive T-cells enhances recruitment of these cells to secondary lymphoid organs by CCL19 and CCL21. Here the solution structure of CCL19 is reported. It contains a canonical chemokine domain. Chemical shift mapping shows the N-termini of PSGL-1 and CCR7 have overlapping binding sites for CCL19 and binding is competitive. Implications for the mechanism of PSGL-1's enhancement of resting T-cell recruitment are discussed.

PubMed: 26115234
DOI: 10.1021/acs.biochem.5b00560

Experimental method

SOLUTION NMR