2MKC

Cooperative Structure of the Heterotrimeric pre-mRNA Retention and Splicing Complex

2MKC の概要

• エントリーDOI: 10.2210/pdb2mkc/pdb
• NMR情報: BMRB: 19766
• 分子名称: U2 snRNP component IST3, Pre-mRNA leakage protein 1, Pre-mRNA-splicing factor CWC26 (3 entities in total)
• 機能のキーワード: spliceosome, snu17p, bud13p, pml1p, heterotrimer, cooperativity, res, splicing, rrm, protein binding, ist3p
• 由来する生物種: Saccharomyces cerevisiae (Baker's yeast); 詳細
• 細胞内の位置: Cytoplasm: P40565 Q07930 P46947
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 19838.95

構造登録者

Wysoczanski, P.,Schneider, C.,Xiang, S.,Munari, F.,Trowitzsch, S.,Wahl, M.C.,Luhrmann, R.,Becker, S.,Zweckstetter, M. (登録日: 2014-02-04, 公開日: 2014-09-03, 最終更新日: 2024-05-15)

主引用文献

Wysoczanski, P.,Schneider, C.,Xiang, S.,Munari, F.,Trowitzsch, S.,Wahl, M.C.,Luhrmann, R.,Becker, S.,Zweckstetter, M.
Cooperative structure of the heterotrimeric pre-mRNA retention and splicing complex.
Nat.Struct.Mol.Biol., 21:911-918, 2014

PubMed Abstract: The precursor mRNA (pre-mRNA) retention and splicing (RES) complex is a spliceosomal complex that is present in yeast and humans and is important for RNA splicing and retention of unspliced pre-mRNA. Here, we present the solution NMR structure of the RES core complex from Saccharomyces cerevisiae. Complex formation leads to an intricate folding of three components-Snu17p, Bud13p and Pml1p-that stabilizes the RNA-recognition motif (RRM) fold of Snu17p and increases binding affinity in tertiary interactions between the components by more than 100-fold compared to that in binary interactions. RES interacts with pre-mRNA within the spliceosome, and through the assembly of the RES core complex RNA binding efficiency is increased. The three-dimensional structure of the RES core complex highlights the importance of cooperative folding and binding in the functional organization of the spliceosome.

PubMed: 25218446
DOI: 10.1038/nsmb.2889

実験手法

SOLUTION NMR