2M5X
Novel method of protein purification for structural research. Example of ultra high resolution structure of SPI-2 inhibitor by X-ray and NMR spectroscopy.
Summary for 2M5X
Entry DOI | 10.2210/pdb2m5x/pdb |
Related | 4hgu |
NMR Information | BMRB: 19085 |
Descriptor | Silk protease inhibitor 2 (1 entity in total) |
Functional Keywords | serine protease inhibitor, kazal family, spi-2, hydrolase inhibitor |
Biological source | Galleria mellonella (Greater wax moth) |
Total number of polymer chains | 1 |
Total formula weight | 4317.74 |
Authors | Lenarcic Zivkovic, M.,Dvornyk, A.,Kludkiewicz, B.,Kopera, E.,Zagorski-Ostoja, W.,Grzelak, K.,Zhukov, I.,Bal, W. (deposition date: 2013-03-12, release date: 2014-03-12, Last modification date: 2023-06-14) |
Primary citation | Kopera, E.,Bal, W.,Lenarcic Zivkovic, M.,Dvornyk, A.,Kludkiewicz, B.,Grzelak, K.,Zhukov, I.,Zagorski-Ostoja, W.,Jaskolski, M.,Krzywda, S. Atomic resolution structure of a protein prepared by non-enzymatic His-tag removal. Crystallographic and NMR study of GmSPI-2 inhibitor. Plos One, 9:e106936-e106936, 2014 Cited by PubMed: 25233114DOI: 10.1371/journal.pone.0106936 PDB entries with the same primary citation |
Experimental method | SOLUTION NMR |
Structure validation
Download full validation report