2M5X
Novel method of protein purification for structural research. Example of ultra high resolution structure of SPI-2 inhibitor by X-ray and NMR spectroscopy.
Spectrometer
Experimental method: SOLUTION NMR
| Spectrometer ID | Spectrometer maker | Spectrometer model | Spectrometer type | Spectrometer field strength |
| 1 | Varian | Uniform NMR System | Varian Uniform NMR System | 800 |
Experiment
| experiment id | conditions id | solution id | Experiment type |
| 1 | 1 | 1 | 2D 1H-1H TOCSY |
| 2 | 1 | 1 | 2D 1H-1H NOESY |
| 3 | 1 | 1 | 2D 1H-15N HSQC |
| 4 | 1 | 1 | 2D 1H-13C HSQC aliphatic |
| 5 | 1 | 1 | 2D 1H-13C HSQC aromatic |
NMR Sample
| conditions id | NMR sample pH | NMR sample pressure | NMR sample temperature |
| 1 | 4.5 | ambient | 298 |
Conformers
| Conformers Calculated Total Number | 200 |
| Conformers Submitted Total Number | 17 |
