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2LZG

NMR Structure of Mdm2 (6-125) with Pip-1

Summary for 2LZG
Entry DOI10.2210/pdb2lzg/pdb
NMR InformationBMRB: 18755
DescriptorE3 ubiquitin-protein ligase Mdm2, [(3R,5R,6S)-5-(3-chlorophenyl)-6-(4-chlorophenyl)-1-(cyclopropylmethyl)-2-oxopiperidin-3-yl]acetic acid (2 entities in total)
Functional Keywordsmdm2, oncogene protein-inhibitor complex, protein binding
Biological sourceHomo sapiens (human)
Cellular locationNucleus, nucleoplasm: Q00987
Total number of polymer chains1
Total formula weight14591.52
Authors
Michelsen, K.B.,Jordan, J.B.,Lewis, J.,Long, A.M.,Yang, E.,Rew, Y.,Zhou, J.,Yakowec, P.,Schnier, P.D.,Huang, X.,Poppe, L. (deposition date: 2012-10-02, release date: 2012-11-07, Last modification date: 2024-05-01)
Primary citationMichelsen, K.,Jordan, J.B.,Lewis, J.,Long, A.M.,Yang, E.,Rew, Y.,Zhou, J.,Yakowec, P.,Schnier, P.D.,Huang, X.,Poppe, L.
Ordering of the N-Terminus of Human MDM2 by Small Molecule Inhibitors.
J.Am.Chem.Soc., 134:17059-17067, 2012
Cited by
PubMed Abstract: Restoration of p53 function through the disruption of the MDM2-p53 protein complex is a promising strategy for the treatment of various types of cancer. Here, we present kinetic, thermodynamic, and structural rationale for the remarkable potency of a new class of MDM2 inhibitors, the piperidinones. While these compounds bind to the same site as previously reported for small molecule inhibitors, such as the Nutlins, data presented here demonstrate that the piperidinones also engage the N-terminal region (residues 10-16) of human MDM2, in particular, Val14 and Thr16. This portion of MDM2 is unstructured in both the apo form of the protein and in MDM2 complexes with p53 or Nutlin, but adopts a novel β-strand structure when complexed with the piperidinones. The ordering of the N-terminus upon binding of the piperidinones extends the current model of MDM2-p53 interaction and provides a new route to rational design of superior inhibitors.
PubMed: 22991965
DOI: 10.1021/ja305839b
PDB entries with the same primary citation
Experimental method
SOLUTION NMR
Structure validation

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