2LK3

U2/U6 Helix I

Summary for 2LK3

• Entry DOI: 10.2210/pdb2lk3/pdb
• Related: 2LKR
• NMR Information: BMRB: 17972
• Descriptor: RNA (5'-R(*GP*GP*CP*UP*UP*AP*GP*AP*UP*CP*AP*GP*AP*AP*AP*UP*GP*AP*UP*CP*AP*GP*CP*C)-3') (1 entity in total)
• Functional Keywords: bulge, rna
• Biological source: Saccharomyces cerevisiae
• Total number of polymer chains: 1
• Total formula weight: 7716.66

Authors

Burke, J.E.,Sashital, D.G.,Zuo, X.E.,Wang, Y.,Butcher, S.E. (deposition date: 2011-10-03, release date: 2012-02-22, Last modification date: 2024-05-15)

Primary citation

Burke, J.E.,Sashital, D.G.,Zuo, X.,Wang, Y.X.,Butcher, S.E.
Structure of the yeast U2/U6 snRNA complex.
Rna, 18:673-683, 2012

PubMed Abstract: The U2/U6 snRNA complex is a conserved and essential component of the active spliceosome that interacts with the pre-mRNA substrate and essential protein splicing factors to promote splicing catalysis. Here we have elucidated the solution structure of a 111-nucleotide U2/U6 complex using an approach that integrates SAXS, NMR, and molecular modeling. The U2/U6 structure contains a three-helix junction that forms an extended "Y" shape. The U6 internal stem-loop (ISL) forms a continuous stack with U2/U6 Helices Ib, Ia, and III. The coaxial stacking of Helix Ib on the U6 ISL is a configuration that is similar to the Domain V structure in group II introns. Interestingly, essential features of the complex--including the U80 metal binding site, AGC triad, and pre-mRNA recognition sites--localize to one face of the molecule. This observation suggests that the U2/U6 structure is well-suited for orienting substrate and cofactors during splicing catalysis.

PubMed: 22328579
DOI: 10.1261/rna.031138.111

Experimental method

SOLUTION NMR