2KZD

Structure of a (3+1) G-quadruplex formed by hTERT promoter sequence

2KZD の概要

• エントリーDOI: 10.2210/pdb2kzd/pdb
• 関連するPDBエントリー: 2A5P 2KZE 2O3M
• 分子名称: DNA (5'-D(*AP*GP*GP*GP*IP*AP*GP*GP*GP*GP*CP*TP*GP*GP*GP*AP*GP*GP*GP*C)-3') (1 entity in total)
• 機能のキーワード: anticancer targets, g-quadruplex, htert promoter, telomerase inhibition, dna
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 6371.09

構造登録者

Lim, K.W.,Lacroix, L.,Yue, D.J.E.,Lim, J.K.C.,Lim, J.M.W.,Phan, A.T. (登録日: 2010-06-16, 公開日: 2010-10-06, 最終更新日: 2024-05-01)

主引用文献

Lim, K.W.,Lacroix, L.,Yue, D.J.E.,Lim, J.K.C.,Lim, J.M.W.,Phan, A.T.
Coexistence of two distinct G-quadruplex conformations in the hTERT promoter
J.Am.Chem.Soc., 132:12331-12342, 2010

PubMed Abstract: The catalytic subunit of human telomerase, hTERT, actively elongates the 3' end of the telomere in most cancer cells. The hTERT promoter, which contains many guanine-rich stretches on the same DNA strand, exhibits an exceptional potential for G-quadruplex formation. Here we show that one particular G-rich sequence in this region coexists in two G-quadruplex conformations in potassium solution: a (3 + 1) and a parallel-stranded G-quadruplexes. We present the NMR solution structures of both conformations, each comprising several robust structural elements, among which include the (3 + 1) and all-parallel G-tetrad cores, single-residue double-chain-reversal loops, and a capping A.T base pair. A combination of NMR and CD techniques, complemented with sequence modifications and variations of experimental condition, allowed us to better understand the coexistence of the two G-quadruplex conformations in equilibrium and how different structural elements conspire to favor a particular form.

PubMed: 20704263
DOI: 10.1021/ja101252n

実験手法

SOLUTION NMR