2KW6
Solution NMR Structure of Cyclin-dependent kinase 2-associated protein 1 (CDK2-associated protein 1; oral cancer suppressor Deleted in oral cancer 1, DOC-1) from H.sapiens, Northeast Structural Genomics Consortium Target Target HR3057H
Summary for 2KW6
| Entry DOI | 10.2210/pdb2kw6/pdb |
| NMR Information | BMRB: 16808 |
| Descriptor | Cyclin-dependent kinase 2-associated protein 1 (1 entity in total) |
| Functional Keywords | structural genomics, northeast structural genomics consortium (nesg), psi-2, protein structure initiative, cancer suppressor, cell cycle |
| Biological source | Homo sapiens (human) |
| Total number of polymer chains | 2 |
| Total formula weight | 14881.08 |
| Authors | Ertekin, A.,Aramini, J.M.,Rossi, P.,Lee, A.B.,Jiang, M.,Ciccosanti, C.T.,Xiao, R.,Swapna, G.V.T.,Rost, B.,Everett, J.K.,Acton, T.B.,Prestegard, J.H.,Montelione, G.T.,Northeast Structural Genomics Consortium (NESG) (deposition date: 2010-03-31, release date: 2010-05-26, Last modification date: 2024-05-15) |
| Primary citation | Ertekin, A.,Aramini, J.M.,Rossi, P.,Leonard, P.G.,Janjua, H.,Xiao, R.,Maglaqui, M.,Lee, H.W.,Prestegard, J.H.,Montelione, G.T. Human cyclin-dependent kinase 2-associated protein 1 (CDK2AP1) is dimeric in its disulfide-reduced state, with natively disordered N-terminal region. J.Biol.Chem., 287:16541-16549, 2012 Cited by PubMed Abstract: CDK2AP1 (cyclin-dependent kinase 2-associated protein 1), corresponding to the gene doc-1 (deleted in oral cancer 1), is a tumor suppressor protein. The doc-1 gene is absent or down-regulated in hamster oral cancer cells and in many other cancer cell types. The ubiquitously expressed CDK2AP1 protein is the only known specific inhibitor of CDK2, making it an important component of cell cycle regulation during G(1)-to-S phase transition. Here, we report the solution structure of CDK2AP1 by combined methods of solution state NMR and amide hydrogen/deuterium exchange measurements with mass spectrometry. The homodimeric structure of CDK2AP1 includes an intrinsically disordered 60-residue N-terminal region and a four-helix bundle dimeric structure with reduced Cys-105 in the C-terminal region. The Cys-105 residues are, however, poised for disulfide bond formation. CDK2AP1 is phosphorylated at a conserved Ser-46 site in the N-terminal "intrinsically disordered" region by IκB kinase ε. PubMed: 22427660DOI: 10.1074/jbc.M112.343863 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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