2JP3
Solution Structure of the human FXYD4 (CHIF) protein in SDS micelles
Summary for 2JP3
| Entry DOI | 10.2210/pdb2jp3/pdb |
| Related | 2jol |
| Descriptor | FXYD domain-containing ion transport regulator 4 (1 entity in total) |
| Functional Keywords | protein, transcription |
| Biological source | Rattus norvegicus (Norway rat) |
| Cellular location | Membrane; Single-pass type I membrane protein (Potential): Q63113 |
| Total number of polymer chains | 1 |
| Total formula weight | 7012.08 |
| Authors | Franzin, C.M.,Teriete, P.,Marassi, F.M. (deposition date: 2007-04-18, release date: 2008-03-04, Last modification date: 2023-12-20) |
| Primary citation | Franzin, C.M.,Teriete, P.,Marassi, F.M. Structural similarity of a membrane protein in micelles and membranes J.Am.Chem.Soc., 129:8078-8079, 2007 Cited by PubMed Abstract: The anisotropic spin interactions measured for membrane proteins in weakly oriented micelles and in oriented lipid bilayers provide independent and potentially complementary high-resolution restraints for structure determination. Here we show that the membrane protein CHIF adopts a similar structure in lipid micelles and bilayers, allowing the restraints from micelle and bilayer samples to be combined in a complementary fashion to enhance the structural information. Back-calculation and assignment of the NMR spectrum of CHIF in oriented lipid bilayers, from the structure determined in micelles, provides additional restraints for structure determination as well as the global orientation of the protein in the membrane. The combined use of solution and solid-state NMR restraints also affords cross-validation for the structural analysis. PubMed: 17567018DOI: 10.1021/ja0728371 PDB entries with the same primary citation |
| Experimental method | SOLUTION NMR |
Structure validation
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