2JM6

Solution structure of MCL-1 complexed with NOXAB

2JM6 の概要

• エントリーDOI: 10.2210/pdb2jm6/pdb
• 関連するPDBエントリー: 1WSX
• 分子名称: Noxa, Myeloid cell leukemia-1 protein Mcl-1 homolog (2 entities in total)
• 機能のキーワード: apoptosis, mcl-1, bcl-2, helical bundle, bh3-only
• 由来する生物種: Mus musculus (house mouse); 詳細
• 細胞内の位置: Mitochondrion: Q9JM54; Membrane; Single-pass membrane protein (By similarity): P97287
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 21405.38

構造登録者

Czabotar, P.E.,Lee, E.F.,van Delft, M.F.,Day, C.L.,Smith, B.J.,Huang, D.C.S.,Fairlie, W.D.,Hinds, M.G.,Colman, P.M. (登録日: 2006-10-17, 公開日: 2007-03-20, 最終更新日: 2023-12-20)

主引用文献

Czabotar, P.E.,Lee, E.F.,van Delft, M.F.,Day, C.L.,Smith, B.J.,Huang, D.C.S.,Fairlie, W.D.,Hinds, M.G.,Colman, P.M.
Structural insights into the degradation of Mcl-1 induced by BH3 domains
Proc.Natl.Acad.Sci.Usa, 104:6217-6222, 2007

PubMed Abstract: Apoptosis is held in check by prosurvival proteins of the Bcl-2 family. The distantly related BH3-only proteins bind to and antagonize them, thereby promoting apoptosis. Whereas binding of the BH3-only protein Noxa to prosurvival Mcl-1 induces Mcl-1 degradation by the proteasome, binding of another BH3-only ligand, Bim, elevates Mcl-1 protein levels. We compared the three-dimensional structures of the complexes formed between BH3 peptides of both Bim and Noxa, and we show that a discrete C-terminal sequence of the Noxa BH3 is necessary to instigate Mcl-1 degradation.

PubMed: 17389404
DOI: 10.1073/pnas.0701297104

実験手法

SOLUTION NMR