2J5X

STRUCTURE OF THE SMALL G PROTEIN ARF6 IN COMPLEX WITH GTPGAMMAS

「1HFV」から置き換えられました

2J5X の概要

• エントリーDOI: 10.2210/pdb2j5x/pdb
• 関連するPDBエントリー: 1E0S 2A5D 2A5F 2A5G
• 分子名称: ADP-RIBOSYLATION FACTOR 6, 5'-GUANOSINE-DIPHOSPHATE-MONOTHIOPHOSPHATE, MAGNESIUM ION, ... (4 entities in total)
• 機能のキーワード: membrane traffic, protein transport, ras, arf, arf6, g protein, myristate, transport, er-golgi transport, nucleotide-binding, lipoprotein, gtp-binding, golgi apparatus
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Golgi apparatus: P62330
• タンパク質・核酸の鎖数: 2
• 化学式量合計: 41347.43

構造登録者

Pasqualato, S.,Menetrey, J.,Franco, M.,Cherfils, J. (登録日: 2006-09-20, 公開日: 2006-09-21, 最終更新日: 2024-05-01)

主引用文献

Pasqualato, S.,Menetrey, J.,Franco, M.,Cherfils, J.
The Structural Gdp-GTP Cycle of Human Arf6.
Embo Rep., 2:234-, 2001

PubMed Abstract: The small GTP-binding protein Arf6 coordinates membrane traffic at the plasma membrane with aspects of cytoskeleton organization. This function does not overlap with that of other members of the ADP-ribosylation factor (Arf) family, although their switch regions, which are their major sites of interaction with regulators and effectors, have virtually identical sequences. Here we report the crystal structure of full-length, non-myristoylated human Arf6 bound to GTPgammaS. Unlike their GDP-bound forms, the active forms of Arf6 and Arf1 are very similar. Thus, the switch regions are discriminatory elements between Arf isoforms in their inactive but not in their active forms, a property that may generalize to other families of small G proteins. This suggests that GTP-bound Arfs may establish specific interactions outside the switch regions and/or be recognized in their cellular context rather than as isolated proteins. The structure also allows further insight into the lack of spontaneous GTPase activity of Arf proteins.

PubMed: 11266366
DOI: 10.1093/EMBO-REPORTS/KVE043

実験手法

X-RAY DIFFRACTION (2.8 Å)