2IUW

Crystal structure of human ABH3 in complex with iron ion and 2- oxoglutarate

2IUW の概要

• エントリーDOI: 10.2210/pdb2iuw/pdb
• 分子名称: ALKYLATED REPAIR PROTEIN ALKB HOMOLOG 3, FE (III) ION, 2-OXOGLUTARIC ACID, ... (5 entities in total)
• 機能のキーワード: oxidoreductase, dna/rna repair, demethylase, beta jellyroll
• 由来する生物種: HOMO SAPIENS (HUMAN)
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 28377.34

構造登録者

Sundheim, O.,Vagbo, C.B.,Bjoras, M.,deSousa, M.M.L.,Talstad, V.,Aas, P.A.,Drablos, F.,Krokan, H.E.,Tainer, J.A.,Slupphaug, G. (登録日: 2006-06-07, 公開日: 2006-07-26, 最終更新日: 2025-04-09)

主引用文献

Sundheim, O.,Vagbo, C.B.,Bjoras, M.,Desousa, M.M.L.,Talstad, V.,Aas, P.A.,Drablos, F.,Krokan, H.E.,Tainer, J.A.,Slupphaug, G.
Human Abh3 Structure and Key Residues for Oxidative Demethylation to Reverse DNA/RNA Damage.
Embo J., 25:3389-, 2006

PubMed Abstract: Methylating agents are ubiquitous in the environment, and central in cancer therapy. The 1-methyladenine and 3-methylcytosine lesions in DNA/RNA contribute to the cytotoxicity of such agents. These lesions are directly reversed by ABH3 (hABH3) in humans and AlkB in Escherichia coli. Here, we report the structure of the hABH3 catalytic core in complex with iron and 2-oxoglutarate (2OG) at 1.5 A resolution and analyse key site-directed mutants. The hABH3 structure reveals the beta-strand jelly-roll fold that coordinates a catalytically active iron centre by a conserved His1-X-Asp/Glu-X(n)-His2 motif. This experimentally establishes hABH3 as a structural member of the Fe(II)/2OG-dependent dioxygenase superfamily, which couples substrate oxidation to conversion of 2OG into succinate and CO2. A positively charged DNA/RNA binding groove indicates a distinct nucleic acid binding conformation different from that predicted in the AlkB structure with three nucleotides. These results uncover previously unassigned key catalytic residues, identify a flexible hairpin involved in nucleotide flipping and ss/ds-DNA discrimination, and reveal self-hydroxylation of an active site leucine that may protect against uncoupled generation of dangerous oxygen radicals.

PubMed: 16858410
DOI: 10.1038/SJ.EMBOJ.7601219

実験手法

X-RAY DIFFRACTION (1.5 Å)