2IM0

Crystal structure of poliovirus polymerase complexed with CTP and Mg2+

Summary for 2IM0

• Entry DOI: 10.2210/pdb2im0/pdb
• Related: 1RA6 1RA7 2ILY 2ILZ 2IM1 2IM2 2IM3
• Descriptor: poliovirus polymerase, SODIUM ION, CYTIDINE-5'-TRIPHOSPHATE, ... (5 entities in total)
• Functional Keywords: nucleotidyltransferase, poliovirus, 3d, rna-dependent, polymerase, ctp, stabilization, transferase
• Biological source: Human poliovirus 1
• Cellular location: Protein VP2: Virion. Protein VP3: Virion. Protein VP1: Virion. Protein 2B: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 2C: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3A: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential). Protein 3B: Virion (Potential). Picornain 3C: Host cytoplasm (Potential). RNA-directed RNA polymerase 3D-POL: Host cytoplasmic vesicle membrane; Peripheral membrane protein; Cytoplasmic side (Potential): P03300
• Total number of polymer chains: 1
• Total formula weight: 53914.37

Authors

Thompson, A.A.,Peersen, O.B. (deposition date: 2006-10-03, release date: 2006-12-12, Last modification date: 2024-10-09)

Primary citation

Thompson, A.A.,Albertini, R.A.,Peersen, O.B.
Stabilization of Poliovirus Polymerase by NTP Binding and Fingers-Thumb Interactions.
J.Mol.Biol., 366:1459-1474, 2007

PubMed Abstract: The viral RNA-dependent RNA polymerases show a conserved structure where the fingers domain interacts with the top of the thumb domain to create a tunnel through which nucleotide triphosphates reach the active site. We have solved the crystal structures of poliovirus polymerase (3D(pol)) in complex with all four NTPs, showing that they all bind in a common pre-insertion site where the phosphate groups are not yet positioned over the active site. The NTPs interact with both the fingers and palm domains, forming bridging interactions that explain the increased thermal stability of 3D(pol) in the presence of NTPs. We have also examined the importance of the fingers-thumb domain interaction for the function and structural stability of 3D(pol). Results from thermal denaturation experiments using circular dichroism and 2-anilino-6-napthaline-sulfonate (ANS) fluorescence show that 3D(pol) has a melting temperature of only approximately 40 degrees C. NTP binding stabilizes the protein and increases the melting by 5-6 degrees C while mutations in the fingers-thumb domain interface destabilize the protein and reduce the melting point by as much as 6 degrees C. In particular, the burial of Phe30 and Phe34 from the tip of the index finger into a pocket at the top of the thumb and the presence of Trp403 on the thumb domain are key interactions required to maintain the structural integrity of the polymerase. The data suggest the fingers domain has significant conformational flexibility and exists in a highly dynamic molten globule state at physiological temperature. The role of the enclosed active site motif as a structural scaffold for constraining the fingers domain and accommodating conformational changes in 3D(pol) and other viral polymerases during the catalytic cycle is discussed.

PubMed: 17223130
DOI: 10.1016/j.jmb.2006.11.070

Experimental method

X-RAY DIFFRACTION (2.25 Å)