2IBT

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position and an abasic site analog at the target base partner position

2IBT の概要

• エントリーDOI: 10.2210/pdb2ibt/pdb
• 関連するPDBエントリー: 1AQI 1AQJ 1G38 2ADM 2IBS
• 分子名称: 5'-D(*GP*TP*TP*CP*GP*(2PR)P*TP*GP*TP*C)-3', 5'-D(*GP*AP*CP*AP*(3DR)P*CP*GP*(6MA)P*AP*C)-3', Modification methylase TaqI, ... (6 entities in total)
• 機能のキーワード: dna, dna methyltransferase, 2-aminopurine, abasic site analog, base flipping, nucleotide flipping, transferase-dna complex, transferase/dna
• 由来する生物種: Thermus aquaticus; 詳細
• タンパク質・核酸の鎖数: 6
• 化学式量合計: 109117.70

構造登録者

Lenz, T.,Scheidig, A.J.,Weinhold, E. (登録日: 2006-09-12, 公開日: 2007-05-29, 最終更新日: 2023-10-25)

• 主引用文献: Lenz, T.,Bonnist, E.Y.M.,Pljevaljcic, G.,Neely, R.K.,Dryden, D.T.F.,Scheidig, A.J.,Jones, A.C.,Weinhold, E.; 2-Aminopurine Flipped into the Active Site of the Adenine-Specific DNA Methyltransferase M.TaqI: Crystal Structures and Time-Resolved Fluorescence; J.Am.Chem.Soc., 129:6240-6248, 2007; PubMed: 17455934; DOI: 10.1021/ja069366n

実験手法

X-RAY DIFFRACTION (1.7 Å)