2IBS

Crystal structure of the adenine-specific DNA methyltransferase M.TaqI complexed with the cofactor analog AETA and a 10 bp DNA containing 2-aminopurine at the target position

Summary for 2IBS

• Entry DOI: 10.2210/pdb2ibs/pdb
• Related: 1AQI 1AQJ 1G38 2ADM 2IBT
• Descriptor: 5'-D(*GP*TP*TP*CP*GP*(2PR)P*TP*GP*TP*C)-3', 5'-D(*GP*AP*CP*AP*TP*CP*GP*(6MA)P*AP*C)-3', Modification methylase TaqI, ... (5 entities in total)
• Functional Keywords: dna, dna methyltransferase, 2-aminopurine, base flipping, nucleotide flipping, transferase-dna complex, transferase/dna
• Biological source: Thermus aquaticus; More
• Total number of polymer chains: 6
• Total formula weight: 108721.23

Authors

Pljevaljcic, G.,Lenz, T.,Scheidig, A.J.,Weinhold, E. (deposition date: 2006-09-12, release date: 2007-05-29, Last modification date: 2023-10-25)

• Primary citation: Lenz, T.,Bonnist, E.Y.M.,Pljevaljcic, G.,Neely, R.K.,Dryden, D.T.F.,Scheidig, A.J.,Jones, A.C.,Weinhold, E.; 2-Aminopurine Flipped into the Active Site of the Adenine-Specific DNA Methyltransferase M.TaqI: Crystal Structures and Time-Resolved Fluorescence; J.Am.Chem.Soc., 129:6240-6248, 2007; PubMed: 17455934; DOI: 10.1021/ja069366n

Experimental method

X-RAY DIFFRACTION (2.4 Å)