2HZK

Crystal structures of a sodium-alpha-keto acid binding subunit from a TRAP transporter in its open form

Summary for 2HZK

• Entry DOI: 10.2210/pdb2hzk/pdb
• Descriptor: TRAP-T family sorbitol/mannitol transporter, periplasmic binding protein, SmoM, GLYCEROL (3 entities in total)
• Functional Keywords: trap transporter, periplasmic subunit, ligand binding, transport protein
• Biological source: Rhodobacter sphaeroides 2.4.1
• Cellular location: Periplasm : Q3J1R2
• Total number of polymer chains: 4
• Total formula weight: 162320.66

Authors

Gonin, S.,Arnoux, P.,Pierru, B.,Alonso, B.,Sabaty, M.,Pignol, D. (deposition date: 2006-08-09, release date: 2007-04-03, Last modification date: 2024-10-30)

Primary citation

Gonin, S.,Arnoux, P.,Pierru, B.,Lavergne, J.,Alonso, B.,Sabaty, M.,Pignol, D.
Crystal structures of an Extracytoplasmic Solute Receptor from a TRAP transporter in its open and closed forms reveal a helix-swapped dimer requiring a cation for alpha-keto acid binding.
Bmc Struct.Biol., 7:11-11, 2007

PubMed Abstract: The import of solutes into the bacterial cytoplasm involves several types of membrane transporters, which may be driven by ATP hydrolysis (ABC transporters) or by an ion or H+ electrochemical membrane potential, as in the tripartite ATP-independent periplasmic system (TRAP). In both the ABC and TRAP systems, a specific periplasmic protein from the ESR family (Extracytoplasmic Solute Receptors) is often involved for the recruitment of the solute and its presentation to the membrane complex. In Rhodobacter sphaeroides, TakP (previously named SmoM) is an ESR from a TRAP transporter and binds alpha-keto acids in vitro.

PubMed: 17362499
DOI: 10.1186/1472-6807-7-11

Experimental method

X-RAY DIFFRACTION (1.7 Å)