2HZK
Crystal structures of a sodium-alpha-keto acid binding subunit from a TRAP transporter in its open form
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2006-02-20 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97940,0.97960,0.97565 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 104.505, 63.826, 127.897 |
Unit cell angles | 90.00, 106.58, 90.00 |
Refinement procedure
Resolution | 60.000 - 1.700 |
R-factor | 0.18 |
Rwork | 0.179 |
R-free | 0.20600 |
Structure solution method | MAD |
RMSD bond length | 0.012 |
RMSD bond angle | 1.309 |
Data reduction software | MOSFLM |
Data scaling software | CCP4 ((SCALA)) |
Phasing software | SOLVE (2.08) |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 60.000 | 1.710 |
High resolution limit [Å] | 1.700 | 1.700 |
Number of reflections | 175128 | |
Completeness [%] | 99.0 | 95.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5.9 | 293 | 100 mM sodium citrate 1.5M ammonium sulfate, pH 5.9, VAPOR DIFFUSION, HANGING DROP, temperature 293K |