2HYI

Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA

Summary for 2HYI

• Entry DOI: 10.2210/pdb2hyi/pdb
• Related: 1p27 2db3 2hxy
• Descriptor: 5'-R(*UP*UP*UP*UP*UP*U)-3', Protein mago nashi homolog, RNA-binding protein 8A, ... (8 entities in total)
• Functional Keywords: exon junction, splicing, mrna processing, translation, dead-box atpase, nonsense mediated decay, hydrolase-rna binding protein-rna complex, hydrolase/rna binding protein/rna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: P61326 Q9Y5S9 P38919; Cytoplasm, perinuclear region: O15234
• Total number of polymer chains: 10
• Total formula weight: 172982.64

Authors

Andersen, C.B.F.,Le Hir, H.,Andersen, G.R. (deposition date: 2006-08-06, release date: 2006-08-15, Last modification date: 2024-02-21)

Primary citation

Andersen, C.B.F.,Ballut, L.,Johansen, J.S.,Chamieh, H.,Nielsen, K.H.,Oliveira, C.L.,Pedersen, J.S.,Seraphin, B.,Le Hir, H.,Andersen, G.R.
Structure of the exon junction core complex with a trapped DEAD-box ATPase bound to RNA.
Science, 313:1968-1972, 2006

PubMed Abstract: In higher eukaryotes, a multiprotein exon junction complex is deposited on spliced messenger RNAs. The complex is organized around a stable core, which serves as a binding platform for numerous factors that influence messenger RNA function. Here, we present the crystal structure of a tetrameric exon junction core complex containing the DEAD-box adenosine triphosphatase (ATPase) eukaryotic initiation factor 4AIII (eIF4AIII) bound to an ATP analog, MAGOH, Y14, a fragment of MLN51, and a polyuracil mRNA mimic. eIF4AIII interacts with the phosphate-ribose backbone of six consecutive nucleotides and prevents part of the bound RNA from being double stranded. The MAGOH and Y14 subunits lock eIF4AIII in a prehydrolysis state, and activation of the ATPase probably requires only modest conformational changes in eIF4AIII motif I.

PubMed: 16931718
DOI: 10.1126/science.1131981

Experimental method

X-RAY DIFFRACTION (2.3 Å)