Loading
PDBj
English日本語简体中文繁體中文한국어
✖
MenuPDBj@FacebookPDBj@X(formerly Twitter)PDBj@BlueSkyPDBj@YouTubewwPDB FoundationwwPDBDonate
RCSB PDBPDBeBMRBAdv. SearchSearch help
SummaryStructural detailsExperimental detailsFunctional detailsSequence NeighborHistoryDownloads

2HYI

Structure of the human exon junction complex with a trapped DEAD-box helicase bound to RNA

Functional Information from GO Data
ChainGOidnamespacecontents
A0000184biological_processnuclear-transcribed mRNA catabolic process, nonsense-mediated decay
A0000381biological_processregulation of alternative mRNA splicing, via spliceosome
A0000398biological_processmRNA splicing, via spliceosome
A0003723molecular_functionRNA binding
A0005515molecular_functionprotein binding
A0005634cellular_componentnucleus
A0005654cellular_componentnucleoplasm
A0005681cellular_componentspliceosomal complex
A0005737cellular_componentcytoplasm
A0005829cellular_componentcytosol
A0006397biological_processmRNA processing
A0006406biological_processmRNA export from nucleus
A0006417biological_processregulation of translation
A0008380biological_processRNA splicing
A0016607cellular_componentnuclear speck
A0035145cellular_componentexon-exon junction complex
A0050684biological_processregulation of mRNA processing
A0051028biological_processmRNA transport
A0071013cellular_componentcatalytic step 2 spliceosome
A1990501cellular_componentexon-exon junction subcomplex mago-y14
A2000622biological_processregulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
B0003676molecular_functionnucleic acid binding
B0003723molecular_functionRNA binding
B0003729molecular_functionmRNA binding
B0005634cellular_componentnucleus
B0005737cellular_componentcytoplasm
B0006396biological_processRNA processing
C0000166molecular_functionnucleotide binding
C0000184biological_processnuclear-transcribed mRNA catabolic process, nonsense-mediated decay
C0000398biological_processmRNA splicing, via spliceosome
C0003676molecular_functionnucleic acid binding
C0003723molecular_functionRNA binding
C0003724molecular_functionRNA helicase activity
C0003729molecular_functionmRNA binding
C0004386molecular_functionhelicase activity
C0005515molecular_functionprotein binding
C0005524molecular_functionATP binding
C0005634cellular_componentnucleus
C0005654cellular_componentnucleoplasm
C0005681cellular_componentspliceosomal complex
C0005730cellular_componentnucleolus
C0005737cellular_componentcytoplasm
C0005829cellular_componentcytosol
C0006364biological_processrRNA processing
C0006397biological_processmRNA processing
C0006406biological_processmRNA export from nucleus
C0006417biological_processregulation of translation
C0008143molecular_functionpoly(A) binding
C0008306biological_processassociative learning
C0008380biological_processRNA splicing
C0010629biological_processnegative regulation of gene expression
C0016020cellular_componentmembrane
C0016607cellular_componentnuclear speck
C0016787molecular_functionhydrolase activity
C0016887molecular_functionATP hydrolysis activity
C0017148biological_processnegative regulation of translation
C0030425cellular_componentdendrite
C0035145cellular_componentexon-exon junction complex
C0035368molecular_functionselenocysteine insertion sequence binding
C0035613molecular_functionRNA stem-loop binding
C0035640biological_processexploration behavior
C0043021molecular_functionribonucleoprotein complex binding
C0043025cellular_componentneuronal cell body
C0045727biological_processpositive regulation of translation
C0048701biological_processembryonic cranial skeleton morphogenesis
C0051028biological_processmRNA transport
C0071006cellular_componentU2-type catalytic step 1 spliceosome
C0071013cellular_componentcatalytic step 2 spliceosome
C0072715biological_processcellular response to selenite ion
C0090394biological_processnegative regulation of excitatory postsynaptic potential
C0098794cellular_componentpostsynapse
C0098978cellular_componentglutamatergic synapse
C0099578biological_processregulation of translation at postsynapse, modulating synaptic transmission
C1904570biological_processnegative regulation of selenocysteine incorporation
C1990416biological_processcellular response to brain-derived neurotrophic factor stimulus
C1990904cellular_componentribonucleoprotein complex
C2000622biological_processregulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
D0003723molecular_functionRNA binding
D0003729molecular_functionmRNA binding
D0006397biological_processmRNA processing
D0035145cellular_componentexon-exon junction complex
G0000184biological_processnuclear-transcribed mRNA catabolic process, nonsense-mediated decay
G0000381biological_processregulation of alternative mRNA splicing, via spliceosome
G0000398biological_processmRNA splicing, via spliceosome
G0003723molecular_functionRNA binding
G0005515molecular_functionprotein binding
G0005634cellular_componentnucleus
G0005654cellular_componentnucleoplasm
G0005681cellular_componentspliceosomal complex
G0005737cellular_componentcytoplasm
G0005829cellular_componentcytosol
G0006397biological_processmRNA processing
G0006406biological_processmRNA export from nucleus
G0006417biological_processregulation of translation
G0008380biological_processRNA splicing
G0016607cellular_componentnuclear speck
G0035145cellular_componentexon-exon junction complex
G0050684biological_processregulation of mRNA processing
G0051028biological_processmRNA transport
G0071013cellular_componentcatalytic step 2 spliceosome
G1990501cellular_componentexon-exon junction subcomplex mago-y14
G2000622biological_processregulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
H0003676molecular_functionnucleic acid binding
H0003723molecular_functionRNA binding
H0003729molecular_functionmRNA binding
H0005634cellular_componentnucleus
H0005737cellular_componentcytoplasm
H0006396biological_processRNA processing
I0000166molecular_functionnucleotide binding
I0000184biological_processnuclear-transcribed mRNA catabolic process, nonsense-mediated decay
I0000398biological_processmRNA splicing, via spliceosome
I0003676molecular_functionnucleic acid binding
I0003723molecular_functionRNA binding
I0003724molecular_functionRNA helicase activity
I0003729molecular_functionmRNA binding
I0004386molecular_functionhelicase activity
I0005515molecular_functionprotein binding
I0005524molecular_functionATP binding
I0005634cellular_componentnucleus
I0005654cellular_componentnucleoplasm
I0005681cellular_componentspliceosomal complex
I0005730cellular_componentnucleolus
I0005737cellular_componentcytoplasm
I0005829cellular_componentcytosol
I0006364biological_processrRNA processing
I0006397biological_processmRNA processing
I0006406biological_processmRNA export from nucleus
I0006417biological_processregulation of translation
I0008143molecular_functionpoly(A) binding
I0008306biological_processassociative learning
I0008380biological_processRNA splicing
I0010629biological_processnegative regulation of gene expression
I0016020cellular_componentmembrane
I0016607cellular_componentnuclear speck
I0016787molecular_functionhydrolase activity
I0016887molecular_functionATP hydrolysis activity
I0017148biological_processnegative regulation of translation
I0030425cellular_componentdendrite
I0035145cellular_componentexon-exon junction complex
I0035368molecular_functionselenocysteine insertion sequence binding
I0035613molecular_functionRNA stem-loop binding
I0035640biological_processexploration behavior
I0043021molecular_functionribonucleoprotein complex binding
I0043025cellular_componentneuronal cell body
I0045727biological_processpositive regulation of translation
I0048701biological_processembryonic cranial skeleton morphogenesis
I0051028biological_processmRNA transport
I0071006cellular_componentU2-type catalytic step 1 spliceosome
I0071013cellular_componentcatalytic step 2 spliceosome
I0072715biological_processcellular response to selenite ion
I0090394biological_processnegative regulation of excitatory postsynaptic potential
I0098794cellular_componentpostsynapse
I0098978cellular_componentglutamatergic synapse
I0099578biological_processregulation of translation at postsynapse, modulating synaptic transmission
I1904570biological_processnegative regulation of selenocysteine incorporation
I1990416biological_processcellular response to brain-derived neurotrophic factor stimulus
I1990904cellular_componentribonucleoprotein complex
I2000622biological_processregulation of nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
J0003723molecular_functionRNA binding
J0003729molecular_functionmRNA binding
J0006397biological_processmRNA processing
J0035145cellular_componentexon-exon junction complex
Functional Information from PDB Data
site_idAC1
Number of Residues6
DetailsBINDING SITE FOR RESIDUE MG C 701
ChainResidue
CTHR89
CASP187
CANP801
CHOH802
CHOH803
CHOH804
site_idAC2
Number of Residues6
DetailsBINDING SITE FOR RESIDUE MG I 702
ChainResidue
IHOH803
IHOH804
IHOH805
ITHR89
IASP187
IANP802
site_idAC3
Number of Residues26
DetailsBINDING SITE FOR RESIDUE ANP C 801
ChainResidue
CPHE58
CLYS60
CGLN65
CSER84
CGLY85
CTHR86
CGLY87
CLYS88
CTHR89
CALA90
CGLU188
CGLY340
CASP342
CARG367
CARG370
CTYR371
CMG701
CHOH803
CHOH805
CHOH810
CHOH812
CHOH837
CHOH859
CHOH866
CHOH887
CHOH947
site_idAC4
Number of Residues23
DetailsBINDING SITE FOR RESIDUE ANP I 802
ChainResidue
IPHE58
ILYS60
IGLN65
ISER84
IGLY85
ITHR86
IGLY87
ILYS88
ITHR89
IALA90
IGLU188
IGLY340
IASP342
IARG367
IARG370
ITYR371
IMG702
IHOH804
IHOH814
IHOH823
IHOH825
IHOH846
IHOH850
Functional Information from PROSITE/UniProt
site_idPS00039
Number of Residues9
DetailsDEAD_ATP_HELICASE DEAD-box subfamily ATP-dependent helicases signature. VLDEADEmL
ChainResidueDetails
CVAL185-LEU193
Functional Information from SwissProt/UniProt
site_idSWS_FT_FI1
Number of Residues156
DetailsDomain: {"description":"RRM","evidences":[{"source":"PROSITE-ProRule","id":"PRU00176","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails
site_idSWS_FT_FI2
Number of Residues340
DetailsDomain: {"description":"Helicase ATP-binding","evidences":[{"source":"PROSITE-ProRule","id":"PRU00541","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails
site_idSWS_FT_FI3
Number of Residues322
DetailsDomain: {"description":"Helicase C-terminal","evidences":[{"source":"PROSITE-ProRule","id":"PRU00542","evidenceCode":"ECO:0000255"}]}
ChainResidueDetails
site_idSWS_FT_FI4
Number of Residues56
DetailsMotif: {"description":"Q motif"}
ChainResidueDetails
site_idSWS_FT_FI5
Number of Residues6
DetailsMotif: {"description":"DEAD box","evidences":[{"evidenceCode":"ECO:0000305"}]}
ChainResidueDetails
site_idSWS_FT_FI6
Number of Residues24
DetailsBinding site: {"evidences":[{"source":"PubMed","id":"16923391","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"16931718","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"19033377","evidenceCode":"ECO:0000269"},{"source":"PubMed","id":"20479275","evidenceCode":"ECO:0000269"},{"source":"PDB","id":"2HYI","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J0Q","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2J0S","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"2XB2","evidenceCode":"ECO:0007744"},{"source":"PDB","id":"3EX7","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI7
Number of Residues2
DetailsModified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P60842","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI8
Number of Residues2
DetailsModified residue: {"description":"Phosphothreonine","evidences":[{"source":"PubMed","id":"18669648","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"20068231","evidenceCode":"ECO:0007744"},{"source":"PubMed","id":"23186163","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI9
Number of Residues2
DetailsModified residue: {"description":"N6-acetyllysine","evidences":[{"source":"UniProtKB","id":"P60843","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI10
Number of Residues4
DetailsModified residue: {"description":"N6-acetyllysine","evidences":[{"source":"PubMed","id":"19608861","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails
site_idSWS_FT_FI11
Number of Residues4
DetailsCross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"UniProtKB","id":"P60842","evidenceCode":"ECO:0000250"}]}
ChainResidueDetails
site_idSWS_FT_FI12
Number of Residues6
DetailsCross-link: {"description":"Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)","evidences":[{"source":"PubMed","id":"28112733","evidenceCode":"ECO:0007744"}]}
ChainResidueDetails

240971

PDB entries from 2025-08-27

PDB statisticsPDBj update infoContact PDBjnumon