2HVY

Crystal structure of an H/ACA box RNP from Pyrococcus furiosus

2HVY の概要

• エントリーDOI: 10.2210/pdb2hvy/pdb
• 分子名称: H/ACA RNA, Probable tRNA pseudouridine synthase B, Small nucleolar rnp similar to gar1, ... (8 entities in total)
• 機能のキーワード: h/aca rna, rnp, pseudouridine synthase, guide rna, isomerase-biosynthetic protein-rna complex, isomerase/biosynthetic protein/rna
• 由来する生物種: Pyrococcus furiosus; 詳細
• タンパク質・核酸の鎖数: 5
• 化学式量合計: 94918.05

構造登録者

Ye, K. (登録日: 2006-07-31, 公開日: 2006-09-12, 最終更新日: 2023-10-25)

主引用文献

Li, L.,Ye, K.
Crystal structure of an H/ACA box ribonucleoprotein particle
Nature, 443:302-307, 2006

PubMed Abstract: H/ACA ribonucleoprotein particles (RNPs) are a family of RNA pseudouridine synthases that specify modification sites through guide RNAs. They also participate in eukaryotic ribosomal RNA processing and are a component of vertebrate telomerases. Here we report the crystal structure, at 2.3 A resolution, of an entire archaeal H/ACA RNP consisting of proteins Cbf5, Nop10, Gar1 and L7ae, and a single-hairpin H/ACA RNA, revealing a modular organization of the complex. The RNA upper stem is bound to a composite surface formed by L7ae, Nop10 and Cbf5, and the RNA lower stem and ACA signature motif are bound to the PUA domain of Cbf5, thereby positioning middle guide sequences so that they are primed to pair with substrate RNA. Furthermore, Gar1 may regulate substrate loading and release. The structure rationalizes the consensus structure of H/ACA RNAs, suggests a functional role of each protein, and provides a framework for understanding the mechanism of RNA-guided pseudouridylation, as well as various cellular functions of H/ACA RNP.

PubMed: 16943774
DOI: 10.1038/nature05151

実験手法

X-RAY DIFFRACTION (2.3 Å)