2HR9

Solution structure of human translationally controlled tumor protein

Replaces:  1Y41

Summary for 2HR9

• Entry DOI: 10.2210/pdb2hr9/pdb
• Descriptor: Translationally-controlled tumor protein (1 entity in total)
• Functional Keywords: tumor protein, apoptosis, metal binding protein
• Biological source: Homo sapiens (human)
• Cellular location: Cytoplasm: P13693
• Total number of polymer chains: 1
• Total formula weight: 20690.50

Authors

Feng, Y.,Liu, D.,Yao, H.,Wang, J. (deposition date: 2006-07-20, release date: 2006-08-08, Last modification date: 2024-05-29)

Primary citation

Feng, Y.,Liu, D.,Yao, H.,Wang, J.
Solution structure and mapping of a very weak calcium-binding site of human translationally controlled tumor protein by NMR
Arch.Biochem.Biophys., 48:57-467, 2007

PubMed Abstract: Human translationally controlled tumor protein (TCTP) is a growth-related, calcium-binding protein. We determined the solution structure and backbone dynamics of human TCTP, and identified the calcium-binding site of human TCTP using multi-dimensional NMR spectroscopy. The overall structure of human TCTP has a rather rigid well-folded core and a very flexible long loop connected by a short two-strand beta-sheet, which shows a conserved fold in the TCTP family. The C-terminal portions of loop L(alpha3beta8) and strand beta9 and the N-terminal region of strand beta8 may form a calcium-binding site in the human TCTP structure, which is largely conserved in the sequence alignment of TCTPs. The K(d) value for the calcium binding is 0.022-0.025 M indicating a very weak calcium-binding site.

PubMed: 17897616
DOI: 10.1016/j.abb.2007.08.021

Experimental method

SOLUTION NMR