2HKI

Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform

Summary for 2HKI

• Entry DOI: 10.2210/pdb2hki/pdb
• Descriptor: Glyceraldehyde-3-phosphate dehydrogenase A, chloroplast, SULFATE ION (2 entities in total)
• Functional Keywords: rossmann fold, apo-form, oxidoreductase
• Biological source: Spinacia oleracea (spinach)
• Cellular location: Plastid, chloroplast: P19866
• Total number of polymer chains: 1
• Total formula weight: 36640.64

Authors

Camara-Artigas, A. (deposition date: 2006-07-04, release date: 2006-11-14, Last modification date: 2023-08-30)

Primary citation

Camara-Artigas, A.,Hirasawa, M.,Knaff, D.B.,Wang, M.,Allen, J.P.
Crystallization and structural analysis of GADPH from Spinacia oleracea in a new form.
Acta Crystallogr.,Sect.F, 62:1087-1092, 2006

PubMed Abstract: Two crystalline forms of GADPH (D-glyceraldehyde-3-phosphate dehydrogenase) from Spinacia oleracea were obtained using sitting-drop vapor diffusion. Despite the very low concentration of GADPH in the solutions, two crystalline forms were obtained, one of which was the previously reported C222 space group with unit-cell parameters a = 155.3, b = 181.7, c = 107.6 A and the other of which belonged to a new space group I4(1)22, with unit-cell parameters a = b = 120.9, c = 154.5 A. Diffraction data were measured from both native and derivatives, yielding structures at a resolution limit of 3.0 A. Differences at the NAD(+)/NADP(+)-binding site seen in these structures compared with the previously reported structure with bound coenzyme suggest that conformational changes associated with pyridine-nucleotide binding may play a role in the regulation of this enzyme.

PubMed: 17077485
DOI: 10.1107/S174430910604200X

Experimental method

X-RAY DIFFRACTION (3 Å)