2HKI
Crystal structure of photosynthetic glyceraldehyde-3-phosphate dehydrogenase A4 isoform
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL7-1 |
| Synchrotron site | SSRL |
| Beamline | BL7-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2001-01-03 |
| Detector | MARRESEARCH |
| Wavelength(s) | 1.008 |
| Spacegroup name | I 41 2 2 |
| Unit cell lengths | 120.805, 120.805, 159.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.21236 |
| Rwork | 0.210 |
| R-free | 0.26644 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1rm4 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.472 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.2.0019) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 15.000 | 3.160 |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.076 | 0.428 |
| Number of reflections | 12035 | |
| <I/σ(I)> | 20.1 | 4.1 |
| Completeness [%] | 99.0 | 100 |
| Redundancy | 8.1 | 8.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 2-5 % PEG 10,000, 0.4-0.6 M lithium sulphate or 0.8-1 M sodium chloride, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 298K |
