2FFY

AmpC beta-lactamase N289A mutant in complex with a boronic acid deacylation transition state analog compound SM3

Summary for 2FFY

• Entry DOI: 10.2210/pdb2ffy/pdb
• Related: 1PI4
• Descriptor: Beta-lactamase, POTASSIUM ION, (1R)-1-(2-THIENYLACETYLAMINO)-1-PHENYLMETHYLBORONIC ACID, ... (5 entities in total)
• Functional Keywords: ampc; beta-lactamase; deacylation; transition state; boronic acid, hydrolase
• Biological source: Escherichia coli
• Cellular location: Periplasm: P00811
• Total number of polymer chains: 2
• Total formula weight: 79813.23

Authors

Chen, Y.,Minasov, G.,Roth, T.A.,Prati, F.,Shoichet, B.K. (deposition date: 2005-12-20, release date: 2006-03-28, Last modification date: 2023-08-30)

• Primary citation: Chen, Y.,Minasov, G.,Roth, T.A.,Prati, F.,Shoichet, B.K.; The deacylation mechanism of AmpC beta-lactamase at ultrahigh resolution; J.Am.Chem.Soc., 128:2970-2976, 2006; PubMed: 16506777; DOI: 10.1021/ja056806m

Experimental method

X-RAY DIFFRACTION (1.07 Å)