2FFH

THE SIGNAL SEQUENCE BINDING PROTEIN FFH FROM THERMUS AQUATICUS

Summary for 2FFH

• Entry DOI: 10.2210/pdb2ffh/pdb
• Related: 1FFH
• Descriptor: PROTEIN (FFH), CADMIUM ION, SULFATE ION (3 entities in total)
• Functional Keywords: ffh, srp54, signal recognition particle, gtpase, m domain, rna-binding, signal sequence-binding, helix-turn-helix, protein targeting, protein transport
• Biological source: Thermus aquaticus
• Total number of polymer chains: 3
• Total formula weight: 142521.11

Authors

Keenan, R.J.,Freymann, D.M.,Walter, P.,Stroud, R.M. (deposition date: 1999-06-29, release date: 1999-07-16, Last modification date: 2023-08-30)

Primary citation

Keenan, R.J.,Freymann, D.M.,Walter, P.,Stroud, R.M.
Crystal structure of the signal sequence binding subunit of the signal recognition particle.
Cell(Cambridge,Mass.), 94:181-191, 1998

PubMed Abstract: The crystal structure of the signal sequence binding subunit of the signal recognition particle (SRP) from Thermus aquaticus reveals a deep groove bounded by a flexible loop and lined with side chains of conserved hydrophobic residues. The groove defines a flexible, hydrophobic environment that is likely to contribute to the structural plasticity necessary for SRP to bind signal sequences of different lengths and amino acid sequence. The structure also reveals a helix-turn-helix motif containing an arginine-rich alpha helix that is required for binding to SRP RNA and is implicated in forming the core of an extended RNA binding surface.

PubMed: 9695947
DOI: 10.1016/S0092-8674(00)81418-X

Experimental method

X-RAY DIFFRACTION (3.2 Å)