2FFH
THE SIGNAL SEQUENCE BINDING PROTEIN FFH FROM THERMUS AQUATICUS
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CHESS BEAMLINE F1 |
| Synchrotron site | CHESS |
| Beamline | F1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-07-04 |
| Detector | PRINCETON 2K |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 195.050, 195.050, 335.720 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 3.200 |
| Rwork | 0.257 |
| R-free | 0.29600 |
| Structure solution method | MIR, MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ffh |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CCP4 |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.360 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Rmerge | 0.089 | 0.360 |
| Number of reflections | 36791 | |
| <I/σ(I)> | 11.3 | 3.1 |
| Completeness [%] | 90.4 | 79.6 |
| Redundancy | 4.1 | 2.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 * | 298 | PROTEIN AT 20 MG/ML IN 5 MM HEPES, PH 7.5. CRYSTALLIZED AT RT BY HANGING DROP VAPOR DIFFUSION USING 1.2 M SODIUM ACETATE, 0.12 M CADMIUM SULFATE, 0.1 M TRIS PH 8.5, 20 MM LITHIUM DODECYL SULFATE, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 20 (mg/ml) | |
| 2 | 1 | drop | HEPES | 5 (mM) | |
| 3 | 1 | reservoir | 1.2 (M) | ||
| 4 | 1 | reservoir | Tris-HCl | 0.1 (M) | |
| 5 | 1 | reservoir | 120 (mM) | ||
| 6 | 1 | reservoir | lithium dodecyl sulfate | 20 (mM) |
