2F8X

Crystal structure of activated Notch, CSL and MAML on HES-1 promoter DNA sequence

Summary for 2F8X

• Entry DOI: 10.2210/pdb2f8x/pdb
• Descriptor: 5'-D(*GP*TP*TP*AP*CP*TP*GP*TP*GP*GP*GP*AP*AP*AP*GP*AP*AP*A)-3', 5'-D(*TP*TP*TP*CP*TP*TP*TP*CP*CP*CP*AP*CP*AP*GP*TP*AP*AP*C)-3', Recombining binding protein suppressor of hairless, isoform 4, ... (6 entities in total)
• Functional Keywords: notch, csl, mastermind, hes-1, ankyrin repeats, rel-homology region, transcription-dna complex, transcription/dna
• Biological source: Homo sapiens (human); More
• Cellular location: Nucleus: Q06330; Cell membrane; Single-pass type I membrane protein (By similarity). Notch 1 intracellular domain: Nucleus (By similarity): P46531; Nucleus speckle: Q92585
• Total number of polymer chains: 5
• Total formula weight: 95742.19

Authors

Nam, Y.,Sliz, P.,Blacklow, S.C. (deposition date: 2005-12-04, release date: 2006-04-04, Last modification date: 2023-08-30)

Primary citation

Nam, Y.,Sliz, P.,Song, L.,Aster, J.C.,Blacklow, S.C.
Structural basis for cooperativity in recruitment of MAML coactivators to Notch transcription complexes.
Cell(Cambridge,Mass.), 124:973-983, 2006

PubMed Abstract: Notch receptors transduce essential developmental signals between neighboring cells by forming a complex that leads to transcription of target genes upon activation. We report here the crystal structure of a Notch transcriptional activation complex containing the ankyrin domain of human Notch1 (ANK), the transcription factor CSL on cognate DNA, and a polypeptide from the coactivator Mastermind-like-1 (MAML-1). Together, CSL and ANK create a groove to bind the MAML-1 polypeptide as a kinked, 70 A helix. The composite binding surface likely restricts the recruitment of MAML proteins to promoters on which Notch:CSL complexes have been preassembled, ensuring tight transcriptional control of Notch target genes.

PubMed: 16530044
DOI: 10.1016/j.cell.2005.12.037

Experimental method

X-RAY DIFFRACTION (3.25 Å)