2F2H

Structure of the YicI thiosugar Michaelis complex

Summary for 2F2H

• Entry DOI: 10.2210/pdb2f2h/pdb
• Descriptor: Putative family 31 glucosidase yicI, SULFATE ION, 4-NITROPHENYL 6-THIO-6-S-ALPHA-D-XYLOPYRANOSYL-BETA-D-GLUCOPYRANOSIDE, ... (6 entities in total)
• Functional Keywords: beta8alpha8 barrel, hydrolase
• Biological source: Escherichia coli
• Total number of polymer chains: 6
• Total formula weight: 536213.89

Authors

Kim, Y.-W.,Lovering, A.L.,Strynadka, N.C.J.,Withers, S.G. (deposition date: 2005-11-16, release date: 2006-02-28, Last modification date: 2023-08-23)

Primary citation

Kim, Y.-W.,Lovering, A.L.,Chen, H.,Kantner, T.,McIntosh, L.P.,Strynadka, N.C.J.,Withers, S.G.
Expanding the Thioglycoligase Strategy to the Synthesis of alpha-linked Thioglycosides Allows Structural Investigation of the Parent Enzyme/Substrate Complex
J.Am.Chem.Soc., 128:2202-2203, 2006

PubMed Abstract: For the first time, the thioglycoligase strategy has been successfully applied to alpha-glycosidases. The alpha-thioglycoligases derived from the family 31 glycosidases, alpha-xylosidase from E. coli (YicI) and alpha-glucosidase from Sulfolobus solfataricus, catalyze thioglycoligase reactions using alpha-glycosyl fluorides and deoxythioglycosides as donors and acceptors, respectively, in yields up to 86%. In addition, we describe the Michaelis complex of YicI using one of the thioglycosides as a nonhydrolyzable substrate analogue and discuss the structural insights this yields into the specificity and mechanism of family 31 alpha-glycosidases and the molecular basis of an associated genetic disease.

PubMed: 16478160
DOI: 10.1021/ja057904a

Experimental method

X-RAY DIFFRACTION (1.95 Å)