2DPJ

structure of hPoli with DNA and dTTP

2DPJ の概要

• エントリーDOI: 10.2210/pdb2dpj/pdb
• 関連するPDBエントリー: 1T3N 2ALZ 2DPI
• 分子名称: 5'-D(*AP*GP*GP*AP*CP*CP*(DOC))-3', 5'-D(*TP*(EDA)P*GP*GP*GP*TP*CP*CP*T)-3', DNA polymerase iota, ... (6 entities in total)
• 機能のキーワード: dna dependent dna polymerase, ethenoda adduct, lesion bypass, dttp, transferase-dna complex, transferase/dna
• 由来する生物種: Homo sapiens (human)
• 細胞内の位置: Nucleus: Q9UNA4
• タンパク質・核酸の鎖数: 3
• 化学式量合計: 52420.37

構造登録者

Nair, D.T.,Johnson, R.E.,Prakash, L.,Prakash, S.,Aggarwal, A.K. (登録日: 2006-05-12, 公開日: 2006-07-04, 最終更新日: 2023-10-25)

主引用文献

Nair, D.T.,Johnson, R.E.,Prakash, L.,Prakash, S.,Aggarwal, A.K.
Hoogsteen base pair formation promotes synthesis opposite the 1,N(6)-ethenodeoxyadenosine lesion by human DNA polymerase iota.
Nat.Struct.Mol.Biol., 13:619-625, 2006

PubMed Abstract: The 1,N6-ethenodeoxyadenosine (epsilon dA) lesion is promutagenic and has been implicated in carcinogenesis. We show here that human Pol iota, a Y-family DNA polymerase, can promote replication through this lesion by proficiently incorporating a nucleotide opposite it. The structural basis of this action is rotation of the epsilon dA adduct to the syn conformation in the Pol iota active site and presentation of its 'Hoogsteen edge' for hydrogen-bonding with incoming dTTP or dCTP. We also show that Pol zeta carries out the subsequent extension reaction and that efficiency of extension from epsilon dA x T is notably higher than from epsilon dA x C. Together, our studies reveal for the first time how the exocyclic epsilon dA adduct is accommodated in a DNA polymerase active site, and they show that the combined action of Pol iota and Pol zeta provides for efficient and error-free synthesis through this potentially carcinogenic DNA lesion.

PubMed: 16819516
DOI: 10.1038/nsmb1118

実験手法

X-RAY DIFFRACTION (2.3 Å)