2DPJ
structure of hPoli with DNA and dTTP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 17-ID |
Synchrotron site | APS |
Beamline | 17-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2005-07-18 |
Detector | ADSC QUANTUM 210 |
Spacegroup name | P 65 2 2 |
Unit cell lengths | 98.059, 98.059, 203.102 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.760 - 2.300 |
R-factor | 0.2412 |
Rwork | 0.238 |
R-free | 0.27700 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2alz |
RMSD bond length | 0.037 |
RMSD bond angle | 2.865 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | REFMAC (5.1.24) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.046 | 0.283 |
Number of reflections | 26535 | |
<I/σ(I)> | 45.6 | 8.9 |
Completeness [%] | 99.3 | |
Redundancy | 10.5 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 16% PEG 5K MME, 0.4M Ammonium Sulfate, 0.1M MES buffer, pH 6.5, VAPOR DIFFUSION, HANGING DROP |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | PEG 5K MME | ||
2 | 1 | 1 | Ammonium Sulfate | ||
3 | 1 | 1 | MES | ||
4 | 1 | 1 | HOH | ||
5 | 1 | 2 | PEG 5K MME | ||
6 | 1 | 2 | Ammonium Sulfate | ||
7 | 1 | 2 | MES | ||
8 | 1 | 2 | HOH |