2D1T

Crystal structure of the thermostable Japanese Firefly Luciferase red-color emission S286N mutant complexed with High-energy intermediate analogue

Summary for 2D1T

Related2D1R 2D1S
DescriptorLuciferin 4-monooxygenase, CHLORIDE ION, 5'-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL] ADENOSINE, ... (4 entities in total)
Functional Keywordsalpha/beta, beta barrel, alpha+beta, riken structural genomics/proteomics initiative, rsgi, structural genomics, oxidoreductase
Biological sourceLuciola cruciata (Japanese firefly)
Total number of polymer chains1
Total molecular weight60837.69
Authors
Nakatsu, T.,Ichiyama, S.,Hiratake, J.,Saldanha, A.,Kobashi, N.,Sakata, K.,Kato, H.,RIKEN Structural Genomics/Proteomics Initiative (RSGI) (deposition date: 2005-08-31, release date: 2006-03-21, Last modification date: 2019-12-25)
Primary citation
Nakatsu, T.,Ichiyama, S.,Hiratake, J.,Saldanha, A.,Kobashi, N.,Sakata, K.,Kato, H.
Structural basis for the spectral difference in luciferase bioluminescence.
Nature, 440:372-376, 2006
PubMed: 16541080 (PDB entries with the same primary citation)
DOI: 10.1038/nature04542
MImport into Mendeley
Experimental method
X-RAY DIFFRACTION (1.45 Å)
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Structure validation

RfreeClashscoreRamachandran outliersSidechain outliersRSRZ outliers 0.16530 1.6% 3.3%MetricValuePercentile RanksWorseBetterPercentile relative to all X-ray structuresPercentile relative to X-ray structures of similar resolution
Download full validation report