2D1T
Crystal structure of the thermostable Japanese Firefly Luciferase red-color emission S286N mutant complexed with High-energy intermediate analogue
Entity
| Entity ID | Chain ID | Description | Type | Chain length | Formula weight | Number of molecules | DB Name (Accession) | Biological source | Descriptive keywords |
| 1 | A (A) | Luciferin 4-monooxygenase | polymer | 548 | 60160.2 | 1 | UniProt (P13129) Pfam (PF00501) Pfam (PF13193) | Luciola cruciata (Japanese firefly) | Luciferase |
| 2 | B, C (A) | CHLORIDE ION | non-polymer | 35.5 | 2 | Chemie (CL) | |||
| 3 | D (A) | 5'-O-[N-(DEHYDROLUCIFERYL)-SULFAMOYL] ADENOSINE | non-polymer | 606.6 | 1 | Chemie (SLU) | |||
| 4 | E (A) | water | water | 18.0 | 843 | Chemie (HOH) |
Sequence modifications
A: 4 - 548 (UniProt: P13129)
| PDB | External Database | Details |
|---|---|---|
| Met 1 | - | deletion |
| Glu 2 | - | deletion |
| Asn 3 | - | deletion |
| Cso 64 | Cys 64 | modified residue |
| Ile 217 | Thr 217 | engineered mutation |
| Asn 286 | Ser 286 | engineered mutation |
Sequence viewer
Contents of the asymmetric unit
| Polymers | Number of chains | 1 |
| Total formula weight | 60160.2 | |
| Non-Polymers* | Number of molecules | 3 |
| Total formula weight | 677.5 | |
| All* | Total formula weight | 60837.7 |
