2CFI

The hydrolase domain of human 10-FTHFD in complex with 6- formyltetrahydropterin

2CFI の概要

• エントリーDOI: 10.2210/pdb2cfi/pdb
• 関連するPDBエントリー: 2BW0 2CQ8
• 分子名称: 10-FORMYLTETRAHYDROFOLATE DEHYDROGENASE, SULFATE ION, 6-FORMYLTETRAHYDROPTERIN, ... (4 entities in total)
• 機能のキーワード: tetrahydrofolate, folate binding, nadp, one-carbon metabolism, oxidoreductase, phosphopantetheine
• 由来する生物種: HOMO SAPIENS (HUMAN)
• 細胞内の位置: Cytoplasm: O75891
• タンパク質・核酸の鎖数: 1
• 化学式量合計: 36866.86

構造登録者

Kursula, P.,Stenmark, P.,Arrowsmith, C.,Edwards, A.,Ehn, M.,Graslund, S.,Hammarstrom, M.,Hallberg, M.,Kotenyova, T.,Nilsson-Ehle, P.,Nordlund, P.,Ogg, D.J.,Persson, C.,Sagemark, J.,Schuler, H.,Sundstrom, M.,Thorsell, A.,Weigelt, J. (登録日: 2006-02-21, 公開日: 2006-03-14, 最終更新日: 2023-12-13)

主引用文献

Kursula, P.,Schuler, H.,Flodin, S.,Nilsson-Ehle, P.,Ogg, D.J.,Savitsky, P.,Nordlund, P.,Stenmark, P.
Structures of the Hydrolase Domain of Human 10-Formyltetrahydrofolate Dehydrogenase and its Complex with a Substrate Analogue.
Acta Crystallogr.,Sect.D, 62:1294-, 2006

PubMed Abstract: 10-Formyltetrahydrofolate dehydrogenase is a ubiquitously expressed enzyme in the human body. It catalyses the formation of tetrahydrofolate and carbon dioxide from 10-formyltetrahydrofolate, thereby playing an important role in the human metabolism of one-carbon units. It is a two-domain protein in which the N-terminal domain hydrolyses 10-formyltetrahydrofolate into formate and tetrahydrofolate. The high-resolution crystal structure of the hydrolase domain from human 10-formyltetrahydrofolate dehydrogenase has been determined in the presence and absence of a substrate analogue. The structures reveal conformational changes of two loops upon ligand binding, while key active-site residues appear to be pre-organized for catalysis prior to substrate binding. Two water molecules in the structures mark the positions of key oxygen moieties in the catalytic reaction and reaction geometries are proposed based on the structural data.

PubMed: 17057331
DOI: 10.1107/S0907444906026849

実験手法

X-RAY DIFFRACTION (1.85 Å)