2CFA
Structure of viral flavin-dependant thymidylate synthase ThyX
Summary for 2CFA
| Entry DOI | 10.2210/pdb2cfa/pdb |
| Descriptor | THYMIDYLATE SYNTHASE, FLAVIN-ADENINE DINUCLEOTIDE, ... (4 entities in total) |
| Functional Keywords | fdts, tscp, flavin dependent thymidylate synthase fad, flavoprotein, nucleotide biosynthesis, methyltransferase, transferase |
| Biological source | PARAMECIUM BURSARIA CHLORELLA VIRUS 1 More |
| Total number of polymer chains | 2 |
| Total formula weight | 51872.35 |
| Authors | Graziani, S.,Bernauer, J.,Skouloubris, S.,Graille, M.,Zhou, C.-Z.,Marchand, C.,Decottignies, P.,van Tilbeurgh, H.,Myllykallio, H.,Liebl, U. (deposition date: 2006-02-17, release date: 2006-05-16, Last modification date: 2024-11-13) |
| Primary citation | Graziani, S.,Bernauer, J.,Skouloubris, S.,Graille, M.,Zhou, C.-Z.,Marchand, C.,Decottignies, P.,Van Tilbeurgh, H.,Myllykallio, H.,Liebl, U. Catalytic Mechanism and Structure of Viral Flavin-Dependent Thymidylate Synthase Thyx. J.Biol.Chem., 281:24048-, 2006 Cited by PubMed Abstract: By using biochemical and structural analyses, we have investigated the catalytic mechanism of the recently discovered flavin-dependent thymidylate synthase ThyX from Paramecium bursaria chlorella virus-1 (PBCV-1). Site-directed mutagenesis experiments have identified several residues implicated in either NADPH oxidation or deprotonation activity of PBCV-1 ThyX. Chemical modification by diethyl pyrocarbonate and mass spectroscopic analyses identified a histidine residue (His53) crucial for NADPH oxidation and located in the vicinity of the redox active N-5 atom of the FAD ring system. Moreover, we observed that the conformation of active site key residues of PBCV-1 ThyX differs from earlier reported ThyX structures, suggesting structural changes during catalysis. Steady-state kinetic analyses support a reaction mechanism where ThyX catalysis proceeds via formation of distinct ternary complexes without formation of a methyl enzyme intermediate. PubMed: 16707489DOI: 10.1074/JBC.M600745200 PDB entries with the same primary citation |
| Experimental method | X-RAY DIFFRACTION (2.3 Å) |
Structure validation
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