2CC2

X-ray crystal structure of 5'-fluorodeoxyadenosine synthase from Streptomyces cattleya complexed with 5'deoxyadenosine

Summary for 2CC2

• Entry DOI: 10.2210/pdb2cc2/pdb
• Related: 1RQP 1RQR 2C2W 2C4T 2C4U 2C5B 2C5H 2CBX
• Descriptor: 5'-FLUORO-5'-DEOXYADENOSINE SYNTHASE, 5'-DEOXYADENOSINE, CHLORIDE ION, ... (4 entities in total)
• Functional Keywords: fluorinase, 5'deoxyadenosine, transferase, fla
• Biological source: STREPTOMYCES CATTLEYA
• Total number of polymer chains: 3
• Total formula weight: 97993.65

Authors

Mcewan, A.R.,Deng, H.,McGlinchey, R.P.,Robinson, D.R.,O'Hagan, D.,Spencer, J.,Naismith, J.H. (deposition date: 2006-01-11, release date: 2007-03-20, Last modification date: 2023-12-13)

Primary citation

Cobb, S.L.,Deng, H.,McEwan, A.R.,Naismith, J.H.,O'Hagan, D.,Robinson, D.A.
Substrate specificity in enzymatic fluorination. The fluorinase from Streptomyces cattleya accepts 2'-deoxyadenosine substrates.
Org. Biomol. Chem., 4:1458-1460, 2006

PubMed Abstract: The fluorinase enzyme from Streptomyces cattleya displays an unusual ability in biocatalysis in that it forms a C-F bond. We now report that the enzyme will accept 2'-deoxyadenosine in place of adenosine substrates, and structural evidence reveals a reorganisation in hydrogen bonding to accommodate this substrate series. It emerges from this study that the enzyme does not require a planar ribose conformation of the substrate to catalyse C-F bond formation.

PubMed: 16604208
DOI: 10.1039/b600574h

Experimental method

X-RAY DIFFRACTION (2 Å)