2B8K

12-subunit RNA Polymerase II

2B8K の概要

• エントリーDOI: 10.2210/pdb2b8k/pdb
• 関連するPDBエントリー: 1WCM
• 分子名称: DNA-directed RNA polymerase II largest subunit, DNA-directed RNA polymerases I/II/III subunit 10, DNA-directed RNA polymerase II 13.6 kDa polypeptide, ... (13 entities in total)
• 機能のキーワード: dna-directed rna polymerase, dna-dependent rna polymerase, cellular rna polymerase, metal-binding, nuclear protein, transcription, transferase
• 由来する生物種: Saccharomyces cerevisiae (baker's yeast); 詳細
• 細胞内の位置: Nucleus: P04050 P38902 P08518 P16370 P20433 P20434 P34087 P20436 P27999; Nucleus, nucleolus: P22139 P40422; Cytoplasm: P20435
• タンパク質・核酸の鎖数: 12
• 化学式量合計: 519749.21

構造登録者

Meyer, P.A.,Ye, P.,Zhang, M.,Suh, M.H.,Fu, J. (登録日: 2005-10-07, 公開日: 2006-06-20, 最終更新日: 2024-10-30)

主引用文献

Meyer, P.A.,Ye, P.,Zhang, M.,Suh, M.H.,Fu, J.
Phasing RNA Polymerase II Using Intrinsically Bound Zn Atoms: An Updated Structural Model.
Structure, 14:973-982, 2006

PubMed Abstract: Macromolecular assemblies as large as RNA polymerase II (Pol II) can be phased by a few intrinsically bound Zn atoms, by using MAD experiments as described here. A phasing effectiveness of 570 aa/Zn is attained for Pol II. The resulting experimental, unbiased electron density map is of such quality that it confirms the existing crystallographic model and further reveals structural regions not shown by model phases, thus updating the Pol II model at three sites. The mechanistically important fork loop-1 element is observed to be ordered in the absence of nucleic acids, suggesting additional insights into the mechanisms that maintain the stability of the transcription ternary complex and allow its release. Furthermore, a computational experiment with simulated MAD data sets demonstrates that 1 Zn site is able to provide adequate experimental phase information for as many as 1100 amino acids of polypeptide, under the conditions of the current synchrotron and detector technologies.

PubMed: 16765890
DOI: 10.1016/j.str.2006.04.003

実験手法

X-RAY DIFFRACTION (4.15 Å)