4V4Q

Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution.

This is a non-PDB format compatible entry.

Summary for 4V4Q

• Entry DOI: 10.2210/pdb4v4q/pdb
• Related: 2AW4 2AW7 2AWB
• Descriptor: 16S ribosomal RNA, 30S ribosomal protein S11, 30S ribosomal protein S12, ... (54 entities in total)
• Functional Keywords: rna-protein complex, 30s ribosomal subunit, ribosome
• Biological source: Escherichia coli; More
• Total number of polymer chains: 104
• Total formula weight: 4293421.75

Authors

Schuwirth, B.S.,Borovinskaya, M.A.,Hau, C.W.,Zhang, W.,Vila-Sanjurjo, A.,Holton, J.M.,Cate, J.H.D. (deposition date: 2005-08-30, release date: 2014-07-09, Last modification date: 2023-09-20)

Primary citation

Schuwirth, B.S.,Borovinskaya, M.A.,Hau, C.W.,Zhang, W.,Vila-Sanjurjo, A.,Holton, J.M.,Cate, J.H.
Structures of the bacterial ribosome at 3.5 A resolution.
Science, 310:827-834, 2005

PubMed Abstract: We describe two structures of the intact bacterial ribosome from Escherichia coli determined to a resolution of 3.5 angstroms by x-ray crystallography. These structures provide a detailed view of the interface between the small and large ribosomal subunits and the conformation of the peptidyl transferase center in the context of the intact ribosome. Differences between the two ribosomes reveal a high degree of flexibility between the head and the rest of the small subunit. Swiveling of the head of the small subunit observed in the present structures, coupled to the ratchet-like motion of the two subunits observed previously, suggests a mechanism for the final movements of messenger RNA (mRNA) and transfer RNAs (tRNAs) during translocation.

PubMed: 16272117
DOI: 10.1126/science.1117230

Experimental method

X-RAY DIFFRACTION (3.46 Å)