4V4Q
Crystal structure of the bacterial ribosome from Escherichia coli at 3.5 A resolution.
This is a non-PDB format compatible entry.
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 12.3.1 |
Synchrotron site | ALS |
Beamline | 12.3.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2004-01-12 |
Detector | ADSC QUANTUM 315 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 208.850, 379.200, 739.300 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 70.000 - 3.460 |
Rwork | 0.279 |
R-free | 0.33100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | pdb entries 1PNS 1PNU |
RMSD bond length | 0.005 |
RMSD bond angle | 1.155 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 70.000 | 3.550 |
High resolution limit [Å] | 3.460 | 3.460 |
Rmerge | 0.145 | 0.594 |
Number of reflections | 693093 | 49172 |
<I/σ(I)> | 7.4 | 2 |
Completeness [%] | 92.3 | 87.9 |
Redundancy | 6.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | batch | 7.5 | 277 | MPD, PEG 8000, MgCl2, NH4Cl, spermine, spermidine, TRIS, EDTA, pH 7.5, batch, temperature 277K |
Crystallization Reagents
ID | crystal ID | solution ID | reagent name | concentration | details |
1 | 1 | 1 | MPD | ||
10 | 1 | 2 | MPD | ||
11 | 1 | 2 | PEG 8000 | ||
12 | 1 | 2 | MgCl2 | ||
13 | 1 | 2 | NH4Cl | ||
2 | 1 | 1 | PEG 8000 | ||
3 | 1 | 1 | MgCl2 | ||
4 | 1 | 1 | NH4Cl | ||
5 | 1 | 1 | spermine | ||
6 | 1 | 1 | spermidine | ||
7 | 1 | 1 | TRIS | ||
8 | 1 | 1 | EDTA | ||
9 | 1 | 1 | H2O |