2A8B

Crystal Structure of the Catalytic Domain of Human Tyrosine Phosphatase Receptor, Type R

Replaces:  1ZEP

Summary for 2A8B

• Entry DOI: 10.2210/pdb2a8b/pdb
• Related: 1JLN
• Descriptor: Receptor-type tyrosine-protein phosphatase R, CHLORIDE ION (3 entities in total)
• Functional Keywords: protein tyrosine phosphatase, receptor, human, structural genomics, structural genomics consortium, sgc, hydrolase
• Biological source: Homo sapiens (human)
• Cellular location: Isoform Alpha: Cell membrane; Single-pass type I membrane protein. Isoform Delta: Cytoplasm, perinuclear region. Isoform Gamma: Cytoplasm, perinuclear region: Q15256
• Total number of polymer chains: 1
• Total formula weight: 32319.49

Authors

Ugochukwu, E.,Eswaran, J.,Barr, A.,Longman, E.,Arrowsmith, C.,Edwards, A.,Sundstrom, M.,von Delft, F.,Knapp, S.,Structural Genomics Consortium (SGC) (deposition date: 2005-07-07, release date: 2005-07-19, Last modification date: 2023-08-23)

Primary citation

Eswaran, J.,von Kries, J.P.,Marsden, B.,Longman, E.,Debreczeni, J.E.,Ugochukwu, E.,Turnbull, A.,Lee, W.H.,Knapp, S.,Barr, A.J.
Crystal structures and inhibitor identification for PTPN5, PTPRR and PTPN7: a family of human MAPK-specific protein tyrosine phosphatases.
Biochem.J., 395:483-491, 2006

PubMed Abstract: Protein tyrosine phosphatases PTPN5, PTPRR and PTPN7 comprise a family of phosphatases that specifically inactivate MAPKs (mitogen-activated protein kinases). We have determined high-resolution structures of all of the human family members, screened them against a library of 24000 compounds and identified two classes of inhibitors, cyclopenta[c]quinolinecarboxylic acids and 2,5-dimethylpyrrolyl benzoic acids. Comparative structural analysis revealed significant differences within this conserved family that could be explored for the design of selective inhibitors. PTPN5 crystallized, in two distinct crystal forms, with a sulphate ion in close proximity to the active site and the WPD (Trp-Pro-Asp) loop in a unique conformation, not seen in other PTPs, ending in a 3(10)-helix. In the PTPN7 structure, the WPD loop was in the closed conformation and part of the KIM (kinase-interaction motif) was visible, which forms an N-terminal aliphatic helix with the phosphorylation site Thr66 in an accessible position. The WPD loop of PTPRR was open; however, in contrast with the structure of its mouse homologue, PTPSL, a salt bridge between the conserved lysine and aspartate residues, which has been postulated to confer a more rigid loop structure, thereby modulating activity in PTPSL, does not form in PTPRR. One of the identified inhibitor scaffolds, cyclopenta[c]quinoline, was docked successfully into PTPRR, suggesting several possibilities for hit expansion. The determined structures together with the established SAR (structure-activity relationship) propose new avenues for the development of selective inhibitors that may have therapeutic potential for treating neurodegenerative diseases in the case of PTPRR or acute myeloblastic leukaemia targeting PTPN7.

PubMed: 16441242
DOI: 10.1042/BJ20051931

Experimental method

X-RAY DIFFRACTION (2.3 Å)