2A8B
Crystal Structure of the Catalytic Domain of Human Tyrosine Phosphatase Receptor, Type R
Replaces: 1ZEPExperimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2005-03-13 |
| Detector | MARMOSAIC 225 mm CCD |
| Wavelength(s) | 0.9184 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 63.193, 74.098, 62.328 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 62.300 - 2.300 |
| R-factor | 0.19515 |
| Rwork | 0.192 |
| R-free | 0.25554 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1JLN.pdb |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.317 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 ((SCALA)) |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.2.0005) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 62.300 | 2.420 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 13031 | |
| Completeness [%] | 97.0 | 98.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | MPD, (NH4)H2PO4, pH 8.5, VAPOR DIFFUSION, SITTING DROP, temperature 277K |
