2A64
Crystal Structure of Bacterial Ribonuclease P RNA
Summary for 2A64
Entry DOI | 10.2210/pdb2a64/pdb |
Descriptor | ribonuclease P RNA (1 entity in total) |
Functional Keywords | hydrolase, ribonuclease, ribozyme, rna |
Total number of polymer chains | 1 |
Total formula weight | 135304.17 |
Authors | Kazantsev, A.V.,Krivenko, A.A.,Harrington, D.J.,Holbrook, S.R.,Adams, P.D.,Pace, N.R. (deposition date: 2005-07-01, release date: 2005-09-20, Last modification date: 2024-02-14) |
Primary citation | Kazantsev, A.V.,Krivenko, A.A.,Harrington, D.J.,Holbrook, S.R.,Adams, P.D.,Pace, N.R. Crystal structure of a bacterial ribonuclease P RNA. Proc.Natl.Acad.Sci.Usa, 102:13392-13397, 2005 Cited by PubMed Abstract: The x-ray crystal structure of a 417-nt ribonuclease P RNA from Bacillus stearothermophilus was solved to 3.3-A resolution. This RNA enzyme is constructed from a number of coaxially stacked helical domains joined together by local and long-range interactions. These helical domains are arranged to form a remarkably flat surface, which is implicated by a wealth of biochemical data in the binding and cleavage of the precursors of transfer RNA substrate. Previous photoaffinity crosslinking data are used to position the substrate on the crystal structure and to identify the chemically active site of the ribozyme. This site is located in a highly conserved core structure formed by intricately interlaced long-range interactions between interhelical sequences. PubMed: 16157868DOI: 10.1073/pnas.0506662102 PDB entries with the same primary citation |
Experimental method | X-RAY DIFFRACTION (3.3 Å) |
Structure validation
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